大麻中 THCA 合酶基因表达分析：实时定量 PCR 的初步研究。
Analysis of THCA synthase gene expression in cannabis: a preliminary study by real-time quantitative PCR.
机构信息
Istituto di Sanità Pubblica, Sezione di Medicina Legale, Università Cattolica del Sacro Cuore, 00168 Roma, Italy.
出版信息
Forensic Sci Int. 2013 Sep 10;231(1-3):208-12. doi: 10.1016/j.forsciint.2013.05.019. Epub 2013 Jun 20.
In this paper we describe analyses performed by Real-Time Reverse-Transcriptase Polymerase Chain Reaction (real-time RT-PCR) on RNA of 12 samples, carried out for forensic purposes to investigate a correlation between tetrahydrocannabinol (THC) concentration in Cannabis and the tetrahydrocannabinol acid synthase (THCAS) gene expression. Samples were obtained from an experimental cultivation of declared potency Cannabis variety seeds and from seizures. The Rubisco gene and the 26S ribosomal RNA gene were used as internal control genes for their constant expression and stability. As results we found minor gene expression in samples from leaves of young plants. Further, grouping results for cannabis samples with similar characteristics, we have found an increased relative expression in samples with the highest percentage of THC coming from seized sample and adult plants.
本文通过实时逆转录聚合酶链反应（real-time RT-PCR）对 12 个样本的 RNA 进行分析，这些样本是为法医学目的而采集的，旨在研究大麻中四氢大麻酚（THC）浓度与四氢大麻酚酸合酶（THCAS）基因表达之间的相关性。样本来自申报效力的大麻品种种子的实验种植和缉获物。Rubisco 基因和 26S 核糖体 RNA 基因被用作内部对照基因，以确保其表达的稳定性。结果发现，年轻植物叶片样本的基因表达水平较低。此外，对具有相似特征的大麻样本进行分组分析，我们发现，来自缉获样本和成年植物的 THC 百分比较高的样本中，相对表达水平有所增加。
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