Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Int J Pharm. 2013 Oct 15;455(1-2):124-31. doi: 10.1016/j.ijpharm.2013.07.048. Epub 2013 Jul 25.
Novel capsules loaded with lactoferrin (LF) were prepared using polyelectrolyte complexes that were formed by water soluble chitosan (WSC), sodium cellulose sulfate (NaCS) and sodium polyphosphate (PPS). Normal chitosan (soluble in acidic conditions) was chosen as a control to prepare similar capsules with NaCS and PPS. (1)H NMR and FTIR spectra analysis showed that WSC was in a form of chitosan hydrochloride which can be directly dissolved and protonated in acid-free water. SEM results showed that the capsules had a typical wall-capsule structure with a regular spherical shape and an average diameter of 1.97 mm. TGA studies revealed that the thermal stability of the capsules were enhanced and the moisture content of the drug-free/loaded capsules were 6.3% and 3.2%. SDS-PAGE results showed that the primary structures of the processed LF in the capsules were unchanged. Drug loading (LE%) and encapsulation efficiency (EE%) analysis showed that the capsules had a higher LE% (45.6%) and EE% (70.7%) than that of the control. In vitro release studies showed that the capsules had a regular and sustainable release profiles in simulated colonic fluid. All of these results indicated that the capsules prepared could be used as a candidate protein drug carrier for colon.
采用聚电解质复合物制备了负载乳铁蛋白（LF）的新型胶囊，聚电解质复合物是由水溶性壳聚糖（WSC）、纤维素硫酸钠（NaCS）和多聚磷酸钠（PPS）形成的。选择普通壳聚糖（可溶于酸性条件下）作为对照，用 NaCS 和 PPS 制备类似的胶囊。（1）H NMR 和 FTIR 光谱分析表明，WSC 是以壳聚糖盐酸盐的形式存在，可以在无酸的水中直接溶解和质子化。SEM 结果表明，胶囊具有典型的壁-胶囊结构，具有规则的球形和平均直径为 1.97mm。TGA 研究表明，胶囊的热稳定性得到提高，无载药/载药胶囊的水分含量分别为 6.3%和 3.2%。SDS-PAGE 结果表明，加工后的 LF 在胶囊中的一级结构保持不变。药物载量（LE%）和包封效率（EE%）分析表明，胶囊的 LE%（45.6%）和 EE%（70.7%）均高于对照。体外释放研究表明，胶囊在模拟结肠液中具有规则和持续的释放曲线。所有这些结果表明，所制备的胶囊可用作结肠蛋白药物载体的候选物。
