Neonatal Brain Research Group, Department of Paediatrics and Child Health, Cork University Maternity Hospital, Wilton, Cork, Ireland.
Clin Biochem. 2013 Dec;46(18):1857-9. doi: 10.1016/j.clinbiochem.2013.07.012. Epub 2013 Jul 24.
The need for early and accurate prediction of outcome in hypoxic-ischaemic encephalopathy (HIE) remains critical. We have previously demonstrated that Interleukin 16 (IL-16) is raised in the umbilical cord blood (UCB) of infants with moderate and severe HIE and has the potential to be developed as a predictive biomarker. Normal reference ranges for IL-16 in UCB have not been previously described. The aim of this study was to determine normative levels of IL-16 in full term neonates using UCB following uncomplicated deliveries and to examine the effect of labour on cord IL-16 values.
Full term infants were recruited as part of an ongoing birth cohort study, the Cork BASELINE Birth Cohort Study. All had UCB drawn and bio-banked at -80°C, within 3hours of birth. Samples for this experiment were chosen from this population based cohort study to represent uncomplicated pre-labour caesarean sections and spontaneous vaginal deliveries. Analysis was performed on plasma EDTA, using ELISA Quantikine® (R&D Systems, Europe).
Samples were analysed from 48 infants with two modes of delivery; spontaneous vaginal delivery (n=12 male, n=12 female) and elective caesarean section (n=12 male, n=12 female). The range of all samples was normally distributed between 87.0 and 114.6pg/ml. Overall mean (SD) for IL-16 was 102.9 (21.5) pg/ml. Levels were not affected by spontaneous vaginal delivery or gender.
For the first time we have described the expected range of cord plasma IL-16 levels in healthy term infants following pre-labour and post-labour delivery.
对缺氧缺血性脑病（HIE）患者进行早期、准确的预后预测仍然至关重要。我们之前已经证明，白细胞介素 16（IL-16）在中重度 HIE 婴儿的脐血（UCB）中升高，并且有可能被开发为预测生物标志物。以前没有描述过 UCB 中 IL-16 的正常参考范围。本研究旨在通过 UCB 确定无并发症分娩后足月新生儿的 IL-16 正常水平，并研究分娩对脐带 IL-16 值的影响。
足月婴儿作为正在进行的出生队列研究（科克 BASELINE 出生队列研究）的一部分被招募。所有婴儿均在出生后 3 小时内采集 UCB 并进行生物样本库储存。本实验的样本取自该人群队列研究，代表无并发症的产前剖宫产和自然阴道分娩。使用 ELISA Quantikine®（R&D Systems，欧洲）分析 EDTA 血浆中的样本。
分析了来自 48 名具有两种分娩方式的婴儿的样本：自然阴道分娩（n=12 名男性，n=12 名女性）和选择性剖宫产（n=12 名男性，n=12 名女性）。所有样本的范围均呈正态分布，在 87.0 至 114.6pg/ml 之间。IL-16 的总体平均值（SD）为 102.9（21.5）pg/ml。水平不受自然阴道分娩或性别影响。
我们首次描述了产前和产后分娩后健康足月婴儿脐带血浆 IL-16 水平的预期范围。
