Abbott Molecular, Des Plaines, IL USA; University of California San Francisco, Department of Community Health, San Francisco, CA, USA.
J Virol Methods. 2013 Nov;193(2):693-6. doi: 10.1016/j.jviromet.2013.07.028. Epub 2013 Jul 25.
The Abbott RealTime (ART) HIV-1 assay targets the integrase region and is designed to tolerate mismatches. Variability in integrase sequences comprising the assay target regions from >1000 clinical specimens submitted for phenotypic and genotypic raltegravir resistance testing were analyzed. In this large collection of sequences from clinical specimens, the number and location of raltegravir resistance associated mutations did not differ from those tested previously and shown not to result in under-estimation of viral loads.
雅培实时（ART）HIV-1 检测法针对整合酶区域，旨在容忍错配。对提交进行表型和基因型拉替拉韦耐药性检测的 >1000 个临床标本中，检测目标区域的整合酶序列的变异性进行了分析。在该临床标本的大型序列集中，拉替拉韦耐药相关突变的数量和位置与之前测试的结果一致，且不会导致病毒载量的低估。
