Department of Occupational and Environmental Hygiene, Chongqing Medical University, People's Republic of China.
Department of Occupational and Environmental Hygiene, Chongqing Medical University, People's Republic of China.
Environ Toxicol Pharmacol. 2013 Sep;36(2):648-658. doi: 10.1016/j.etap.2013.06.008. Epub 2013 Jun 28.
Benzo(a)pyrene (B[a]P) is an environmental carcinogen that induces tumors in many animal species, but the neurotoxic effects of B[a]P have not been well studied. In the present study, we investigated the effects of subacute exposure to B[a]P in Sprague-Dawley (SD) rats. Male rats received daily injections of either B[a]P (0, 1, 2.5, or 6.25mg/kg, i.p.) or vehicle for 45 days. Exposure to B[a]P affected the behavior of rats in the Morris water maze test. Gene microarray and real-time PCR analyses revealed that exposure to B[a]P affected signal transduction in the rat hippocampus. Protein microarray analysis revealed that altered protein expression played a role in cell death in the functional annotation cluster analysis. Finally, major vault protein was found to display low cDNA and protein expression levels. The present study explored some of the possible mechanisms underlying B[a]P neurotoxicity and provided evidence that B[a]P plays a neurotoxic role in rats.
苯并(a)芘（B[a]P）是一种环境致癌物，可在许多动物物种中诱导肿瘤，但 B[a]P 的神经毒性作用尚未得到很好的研究。在本研究中，我们研究了亚急性 B[a]P 暴露对 Sprague-Dawley（SD）大鼠的影响。雄性大鼠每天接受腹腔注射 B[a]P（0、1、2.5 或 6.25mg/kg）或载体 45 天。B[a]P 暴露影响了大鼠在 Morris 水迷宫测试中的行为。基因微阵列和实时 PCR 分析显示，B[a]P 暴露影响了大鼠海马中的信号转导。蛋白质微阵列分析显示，功能注释聚类分析中细胞死亡相关的差异蛋白表达起作用。最后，发现主要穹窿蛋白的 cDNA 和蛋白表达水平较低。本研究探讨了 B[a]P 神经毒性的一些可能机制，并提供了 B[a]P 对大鼠具有神经毒性作用的证据。
