Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huangzhou, China.
J Sep Sci. 2013 Oct;36(19):3264-9. doi: 10.1002/jssc.201300464. Epub 2013 Aug 23.
An MEKC method with LIF detection has been developed for the determination of seven neurotransmitter amino acids (NAAs) using 1,3,5,7-tetramethyl-8-(N-hydroxysuccinimidyl butyric ester)difluoroboradiaza-S-indacene as the labeling reagent. After derivatization at room temperature for 30 min, the seven target NAAs including glycine, alanine, γ-aminobutyric acid, taurine, glutamine, glutamic acid, and aspartic acid were separated in running buffer, which consisted of 70 mM pH 4.00 H3 PO4 /Na3 PO4 buffer, 5.5 mM cetyltrimethyl ammonium bromide and 20% v/v acetonitrile within 17 min. The LODs were 2 ~ 14 × 10(-10) M without interference from other coexisting amino acids. The proposed method has been applied to the analysis of NAAs in the central nervous systems of healthy mice and those with Alzheimer's disease with recoveries of 92-104%.
建立了一种 MEKC 方法，使用 1,3,5,7-四甲基-8-(N-羟基琥珀酰亚胺丁酸酯)二氟硼-aza-S-吲哚作为标记试剂，用于测定七种神经递质氨基酸 (NAAs)。室温衍生 30 分钟后，七种目标 NAAs（甘氨酸、丙氨酸、γ-氨基丁酸、牛磺酸、谷氨酰胺、谷氨酸和天冬氨酸）在运行缓冲液中得到分离，运行缓冲液由 70 mM pH 4.00 H3 PO4/Na3 PO4 缓冲液、5.5 mM 十六烷基三甲基溴化铵和 20% v/v 乙腈组成，在 17 分钟内完成分离。在没有其他共存氨基酸干扰的情况下，LOD 为 2~14×10(-10) M。该方法已应用于健康小鼠和阿尔茨海默病小鼠中枢神经系统中 NAAs 的分析，回收率为 92-104%。
