Kido Hueliton Wilian, Ribeiro Daniel Araki, de Oliveira Poliani, Parizotto Nivaldo Antônio, Camilo Claudia Cristiane, Fortulan Carlos Alberto, Marcantonio Elcio, da Silva Victor Hugo Pereira, Renno Ana Claudia Muniz
Department of Physiotherapy, Post-Graduate Program of Biotechnology, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
J Biomed Mater Res A. 2014 Jul;102(7):2072-8. doi: 10.1002/jbm.a.34877. Epub 2013 Jul 30.
This study aimed to evaluate the osteointegration and genotoxic potential of a bioactive scaffold, composed of alumina and coated with hydroxyapatite and bioglass, after their implantation in tibias of rats. For this purpose, Wistar rats underwent surgery to induce a tibial bone defect, which was filled with the bioactive scaffolds. Histology analysis (descriptive and morphometry) of the bone tissue and the single-cell gel assay (comet) in multiple organs (blood, liver, and kidney) were used to reach this aim after a period of 30, 60, 90, and 180 days of material implantation. The main findings showed that the incorporation of hydroxyapatite and bioglass in the alumina scaffolds produced a suitable environment for bone ingrowth in the tibial defects and did not demonstrate any genotoxicity in the organs evaluated in all experimental periods. These results clearly indicate that the bioactive scaffolds used in this study present osteogenic potential and still exhibit local and systemic biocompatibility. These findings are promising once they convey important information about the behavior of this novel biomaterial in biological system and highlight its possible clinical application.
本研究旨在评估一种由氧化铝组成、涂覆有羟基磷灰石和生物玻璃的生物活性支架植入大鼠胫骨后其骨整合和遗传毒性潜力。为此，Wistar大鼠接受手术以诱导胫骨骨缺损，并用生物活性支架填充。在材料植入30、60、90和180天后，使用骨组织的组织学分析（描述性和形态计量学）以及多个器官（血液、肝脏和肾脏）的单细胞凝胶试验（彗星试验）来实现这一目标。主要研究结果表明，在氧化铝支架中加入羟基磷灰石和生物玻璃为胫骨缺损处的骨生长创造了适宜的环境，并且在所有实验阶段评估的器官中均未表现出任何遗传毒性。这些结果清楚地表明，本研究中使用的生物活性支架具有成骨潜力，并且仍然表现出局部和全身生物相容性。这些发现很有前景，因为它们传达了关于这种新型生物材料在生物系统中行为的重要信息，并突出了其可能的临床应用。
