Li Yong, Yang Xiao-Wei, Dong Xiao-Xiao, Chen Xiao-Jun, Xu Zhi-Peng, Zhang Wei-Wei, Xue Xue, Kong Wen-Jun, Zhou Sha, Zhu Ji-Feng, Liu Feng, Su Chuan
Department of Pathogen Biology, Nanjing Medical University, Nanjing 210029, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Apr;25(2):141-5.
To study the dynamics of IL-22-producing cells and analyze the main source of IL-22 of mice infected with Schistosoma japonicum.
The lymphocytes and splenocytes were prepared from lymphnodes and spleens of mice with S. japonicum infection at different stages (0 w, 3 w, 5w, 8 w, 13 w after infection). The proportions of IL-22-producing cells were determined by FACS.
The proportions of IL-22-producing cells increased significantly in the lymphocytes and splenocytes from the mice infected with S. japonicum. The IL-22-producing T cells accounted 80% of the total IL-22-producing cells. The IL-22-producing CD4- T cells increased obviously in the 3rd week after S. japonicum infection. However, the proportion of the IL-22-producing CD4+ T cells began to rise since the 3rd week post-infection, and increased significantly at the 8th week after infection.
In the lymphnodes and spleens of mice infected with S. japonicum, IL-22 is produced by both T cells and non-T cells, and T cells are the most important source of IL-22. The IL-22-producing CD4- T cells increase rapidly in the early acute inflammatory response, and the IL-22-producing CD4+ T cells increase significantly during the chronic inflammatory phase.
研究日本血吸虫感染小鼠中产生白细胞介素-22（IL-22）细胞的动态变化，并分析IL-22的主要来源。
从小鼠感染日本血吸虫不同阶段（感染后0周、3周、5周、8周、13周）的淋巴结和脾脏中制备淋巴细胞和脾细胞。通过流式细胞术（FACS）测定产生IL-22细胞的比例。
感染日本血吸虫小鼠的淋巴细胞和脾细胞中产生IL-22细胞的比例显著增加。产生IL-22的T细胞占产生IL-22细胞总数的80%。感染日本血吸虫后第3周，产生IL-22的CD4 - T细胞明显增加。然而，产生IL-22的CD4+ T细胞比例自感染后第3周开始上升，并在感染后第8周显著增加。
在感染日本血吸虫的小鼠淋巴结和脾脏中，T细胞和非T细胞均可产生IL-22，且T细胞是IL-22的最重要来源。产生IL-22的CD4 - T细胞在早期急性炎症反应中迅速增加，而产生IL-22的CD4+ T细胞在慢性炎症期显著增加。
