Jia Yu-Xi, Nie Da-Ping, Chen Ling-Juan, Li Wei, Shen Li-Jie
Department of Parasitology, College of Basic Medical, Dali University, Dali 671003, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Apr;25(2):202-4.
Toxoplasma gondii is a nucleated cell obligate parasite, and can cause severe toxoplasmosis. The genetypes of Toxoplasma gondii isolates of different host infections have significant differences, and the pathogenicity and sensitivity to drugs of different genotypes of Toxoplasma gondii isolates are also significantly different. At present, the analysis of Toxoplasma gondii genotypes is performed by using PCR-RFLP, PCR of highly repetitive sequences, multiple PCR and nested PCR techniques, and the multiple loci of amplification are usually B1, SAG2, HSP70, GRA6 and other genetic markers. There are main 3 traditional genotypes of Toxoplasma gondii, and along with the deepening and extension of research, more and more genotypes are found. This paper reviews the advances in the research of Toxoplasma gondii genotypes.
弓形虫是一种有核细胞专性寄生虫，可引起严重的弓形虫病。不同宿主感染的弓形虫分离株的基因型存在显著差异，不同基因型的弓形虫分离株的致病性和对药物的敏感性也有显著差异。目前，弓形虫基因型分析采用PCR-RFLP、高度重复序列PCR、多重PCR和巢式PCR技术进行，扩增的多个位点通常是B1、SAG2、HSP70、GRA6等遗传标记。弓形虫主要有3种传统基因型，随着研究的深入和拓展，发现的基因型越来越多。本文综述了弓形虫基因型的研究进展。
