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[牧豆树属(Prosopisferox)和羽叶豆属(Pterogyne nitens)(豆科)种子的耐旱性]

[Desiccation tolerance in seeds of Prosopisferox and Pterogyne nitens (Fabaceae)].

作者信息

Morandini Marcelo Nahuel, Giamminola Eugenia Mabel, de Viana Marta Leonor

机构信息

Banco de Germoplasma de Especies Nativas del Instituto de Ecologia y Ambiente Humano (BGEN-INEAH), Universidad Nacional de Salta, Avenida Bolivia 5150, Salta CP 4400, Argentina.

出版信息

Rev Biol Trop. 2013 Mar;61(1):335-42.

Abstract

The high number of endemisms and species diversity together with the accelerated biodiversity loss by deforestation, especially in North Western Argentina, points out the need to work on species conservation combining ex situ and in situ strategies. The aim of this work was to study the desiccation tolerance in seeds of P ferox and P nitens for long term ex situ conservation at the Germplasm Bank of Native Species (BGEN) of the National University of Salta (Argentina). The fruits were collected from ten individuals in P ferox at the National Park Los Cardones and from two sites (Orán and Rivadavia) for P nitens. Desiccation tolerance was assessed following previous established methodologies. The moisture content (MC) of the seeds was determined by keeping them in oven at 103 degreeC and weighting the samples at different intervals till constant weight. Germination essays were carried out with two treatments (control and scarification), with different seed MC (fresh, 10-12%, 3-5%) and in desiccated seeds (3-5% MC) stored six months at -20 degreeC. The MC in P ferox seeds was 14.2% and 10% in P nitens, for both populations studied. Percentage germination in P ferox was higher in the scarification treatments (<82%). The difference between treatments increased with the reduction in MC and the storage for six months at -20 degreeC. Fresh seeds of P nitens do not need scarification treatment, but it is required with the reduction in MC and storage. Mean germination percentage of desiccated seeds stored six months at -20 degreeC was similar in both populations and greater than 82%.We concluded that both species are probably orthodox because seeds tolerated desiccation to 3-5% and storage for six months at -20 degree C.

摘要

大量的特有物种和物种多样性,再加上森林砍伐导致生物多样性加速丧失,尤其是在阿根廷西北部,这表明需要结合迁地保护和就地保护策略来开展物种保护工作。这项工作的目的是研究粗茎针茅(P ferox)和柔枝针茅(P nitens)种子的耐干燥性,以便在阿根廷萨尔塔国立大学的本地物种种质库(BGEN)进行长期迁地保护。果实从洛斯卡尔多内斯国家公园的10株粗茎针茅个体以及柔枝针茅的两个地点(奥兰和里瓦达维亚)采集。按照先前确立的方法评估耐干燥性。通过将种子置于103℃的烘箱中,并在不同时间间隔称重直至恒重,来测定种子的含水量(MC)。发芽试验采用两种处理方式(对照和划破种皮),设置不同的种子含水量(新鲜种子、10 - 12%、3 - 5%),并对含水量为3 - 5%的干燥种子在-20℃下储存6个月后进行试验。在所研究的两个种群中,粗茎针茅种子的含水量为14.2%,柔枝针茅种子的含水量为10%。粗茎针茅在划破种皮处理中的发芽率较高(<82%)。随着含水量降低以及在-20℃下储存6个月,处理之间的差异增大。柔枝针茅的新鲜种子不需要划破种皮处理,但随着含水量降低和储存则需要。在-20℃下储存6个月的干燥种子的平均发芽率在两个种群中相似且大于82%。我们得出结论,这两个物种的种子可能都是正统种子,因为它们能耐受干燥至含水量3 - 5%并在-20℃下储存6个月。

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