Department of Physiology, Basic Medical College, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Am J Chin Med. 2013;41(4):789-800. doi: 10.1142/S0192415X13500535.
Ginkgo biloba has been used for medical purposes for centuries in traditional Chinese medicine. Ginkgo biloba extract 50 (GBE50) is a new standardized GBE product that matches the standardized German product as EGb761. This paper is aimed at studying the cardio-protection effects of GBE50 Salvia miltiorrhiza on myocardial function, area at risk, myocardial ultra-structure, and expression of calcium handling proteins in rat ischemic myocardium. Myocardium ischemia was induced by the left anterior descending (LAD) coronary artery occlusion and myocardial function was recorded by a transducer advanced into the left ventricle on a computer system. In vitro myocardial infarction was measured by 2,3,5-triphenyltetrazolium chloride (TTC) and Evans blue staining of heart sections. Morphological change was evaluated by electric microscopy and Western blotting was used for protein expression. Hemodynamic experiments in vivo showed that postischemic cardiac contractile function was reduced in ischemic rats. Salvia miltiorrhiza (7.5 g/kg/d×7) and Ginkgo biloba extract 50 (GBE50) (100 mg/kg/d×7) improved post-schemic cardiac diastolic dysfunction while not affecting the systolic function. In hearts of GBE50 group and Salvia miltiorrhiza (SM) group, the area at risk was significantly reduced and myocardial structure was better-preserved. Moreover, Na⁺-Ca²⁺ exchanger (NCX) expression increase and sarcoplasmic reticulum Ca²⁺-ATPase 2 (SERCA2), LTCC, and ryanodine receptor 2 (RyR2) expression decreases were smaller than those in ischemia group. There was a significant difference between the GBE50 and ischemia group in NCX expression. GBE50 could improve recovery in contractile function and prevent myocardium from ischemia damage, which may be caused by attenuating the abnormal expression of NCX.
银杏叶在传统中药中已被用于医疗用途数百年。银杏叶提取物 50（GBE50）是一种新的标准化 GBE 产品，与标准化的德国产品 EGb761 相匹配。本文旨在研究银杏叶提取物 50 丹参对大鼠缺血心肌功能、危险区面积、心肌超微结构和钙处理蛋白表达的心脏保护作用。通过左前降支（LAD）冠状动脉闭塞诱导心肌缺血，通过将换能器插入计算机系统中的左心室记录心肌功能。通过 2,3,5-三苯基氯化四氮唑（TTC）和心脏切片的 Evans 蓝染色测量体外心肌梗死。通过电子显微镜评估形态变化，并使用 Western blot 进行蛋白表达。体内血液动力学实验表明，缺血大鼠缺血后心脏收缩功能降低。丹参（7.5 g/kg/d×7）和银杏叶提取物 50（GBE50）（100 mg/kg/d×7）改善了缺血后舒张功能障碍，而不影响收缩功能。在 GBE50 组和丹参（SM）组的心脏中，危险区面积明显减少，心肌结构保存更好。此外，Na ⁺ -Ca ²⁺ 交换体（NCX）表达增加，肌浆网 Ca ²⁺ -ATP 酶 2（SERCA2）、LTCC 和兰尼碱受体 2（RyR2）表达减少均小于缺血组。NCX 表达在 GBE50 组和缺血组之间存在显著差异。GBE50 可改善收缩功能的恢复并防止心肌缺血损伤，这可能是通过减轻 NCX 的异常表达引起的。
