Jupin I, Bouzoubaa S, Richards K, Jonard G, Guilley H
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Virology. 1990 Sep;178(1):281-4. doi: 10.1016/0042-6822(90)90404-f.
Beet necrotic yellow vein virus RNAs 1 and 2 but not RNAs 3 and 4 are required for viral multiplication in Chenopodium quinoa leaves. Elimination of the 3' poly(A) tail from RNA 3 transcripts markedly attenuated their ability to be amplified when co-inoculated with RNAs 1 and 2 to this host. Successful multiplication of the tailless RNA 3 was accompanied by the reappearance of new 3' poly(A) tails on the progeny. The evidence suggests that the newly acquired poly(A) sequence results from the action of a poly(A) polymerase rather than recombination with the homologous 3' terminal domains of RNAs 1 or 2. An unexpected feature of these progeny RNA 3 molecules was the presence of a novel short heterogenous U-rich tract separating the poly(A) tail from the 3' end of the heteropolymeric RNA 3 sequence proper.
藜麦坏死黄脉病毒的RNA 1和RNA 2而非RNA 3和RNA 4是该病毒在藜麦叶片中增殖所必需的。从RNA 3转录本中去除3' poly(A)尾，当与RNA 1和RNA 2共同接种到该宿主时，显著减弱了它们被扩增的能力。无尾RNA 3的成功增殖伴随着子代上重新出现新的3' poly(A)尾。证据表明，新获得的poly(A)序列是由poly(A)聚合酶的作用产生的，而不是与RNA 1或RNA 2的同源3'末端结构域重组产生的。这些子代RNA 3分子的一个意外特征是存在一个新的短异质富含U的区域，该区域将poly(A)尾与异聚RNA 3序列本身的3'末端分隔开。
