Qi Cunfang, Zhang Junfeng, Chen Xinlin, Zhang JiansHui, Yang Pengbo, Jiao Qian, Zhang Pengbo, Lu Hai-Xia, Liu Yong
Xi'an Jiaotong University College of Medicine, Xi'an, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jul;33(7):962-6.
To explore the effects of salidroside (sal) on the expressions of Bcl-2, Bax and caspases-3 proteins in cultured rat subventricular zone (SVZ) neural stem cells (NSCs) exposed to hypoxia injury.
Primarily cultured SVZ NSCs from adult SD rats were incubated with salidroside (120 and 240 µmol/L) for 24 h prior to exposure to hypoxia. The cell viability was assessed with MTT assay, and the cell apoptosis was analyzed using TUNEL staining and flow cytometry. Western blotting was performed to detect the expressions of Bcl-2, Bax and caspase-3 in the cells.
Salidroside pretreatment of the cells for 24 h resulted in an obvious resistance to hypoxia-induced cell apoptosis and decrement of cell viability (P<0.05). Salidroside also antagonized the effect of hypoxia exposure in lowering Bcl-2/Bax ratio apoptosis of rat neural stem cells and decreased the expression of caspases-3 protein (P<0.05).
Salidroside can significantly resist hypoxia-induced. The neuroprotective effect of salidroside may be related to the modulation of expressions of apoptosis-related proteins.
探讨红景天苷（sal）对缺氧损伤的成年大鼠脑室下区（SVZ）神经干细胞（NSCs）中Bcl-2、Bax和半胱天冬酶-3蛋白表达的影响。
成年SD大鼠原代培养的SVZ神经干细胞，在缺氧处理前用红景天苷（120和240 μmol/L）孵育24小时。采用MTT法评估细胞活力，并用TUNEL染色和流式细胞术分析细胞凋亡情况。通过蛋白质印迹法检测细胞中Bcl-2、Bax和半胱天冬酶-3的表达。
红景天苷预处理细胞24小时可明显抵抗缺氧诱导的细胞凋亡并减少细胞活力的降低（P<0.05）。红景天苷还拮抗了缺氧暴露对大鼠神经干细胞Bcl-2/Bax比值降低和半胱天冬酶-3蛋白表达减少的影响（P<0.05）。
红景天苷能显著抵抗缺氧诱导。红景天苷的神经保护作用可能与凋亡相关蛋白表达的调节有关。
