Chai Ningli, Zhang Wencheng, Wang Yanmin, Zhou Zhaotao, Zhang Yane, Liu Hongyan, Wan Jun, Qin Jinhua, Wang Shuyong, Wang Yunfang, Pei Xuetao, Wu Benyan, Linghu Enqiang
Department of Gastroenterology, General Hospital of PLA, Beijing, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jul;33(7):972-6.
To study the expression of tumorigenesis-related stem cell markers Lgr5 and CD44 in different pathological types of intestinal polyps and their clinical significance in predicting tumorigenesis.
A total of 145 cases of colorectal polyps, adenomas and cancer tissues were obtained by colonoscopy biopsy. Immunohistochemistry was employed to detect the expression of Lgr5 and CD44 to analyze their relationship with the occurrence and prognosis of colon and rectal cancer.
The expression of CD44 in colon cancer tissue was 95.65%, significantly higher than that in normal mucosa (5%), inflammatory hyperplastic polyps (22.58%), tubular adenomatous polyps (55.26%) and villous polyps (75.76%) (P<0.05). The expression of Lgr5 in colorectal cancer was up to 95.65% while negative in normal colorectal tissue and was 16.12% in inflammatory hyperplastic tissues (P<0.05). The expression rate of Lgr5 was 86.84% in tubular adenoma and 93.94% in villous polyps, both comparable with that in colon cancer (P>0.05). Correlation analysis indicated that the expression of CD44 and Lgr5 were positively correlated with the progression of intestinal polyp tumorigenesis (rs=0.69377, P<0.0001; rs=0.81637, P<0.0001).
Lgr5 and CD44 are highly expressed in colorectal cancer tissues in close correlation with the clinical and pathological features. The expression profiles of Lgr5 and CD44 represent a distinct feature to differentiate colorectal cancer from normal intestinal mucosa. Lgr5 is more closely correlated with tumor progression of polyps than CD44. This means detecting of the expression of Lgr 5 together with CD44 is important and necessary in clinical diagnosis of patients with early stage colorectal diseases such as polyps and their canceration.
研究肿瘤发生相关干细胞标志物Lgr5和CD44在不同病理类型肠息肉中的表达情况及其在预测肿瘤发生中的临床意义。
通过结肠镜活检获取145例结直肠息肉、腺瘤及癌组织。采用免疫组织化学法检测Lgr5和CD44的表达，分析其与结直肠癌发生及预后的关系。
CD44在结肠癌组织中的表达率为95.65%，显著高于正常黏膜（5%）、炎性增生性息肉（22.58%）、管状腺瘤性息肉（55.26%）和绒毛状息肉（75.76%）（P<0.05）。Lgr5在结直肠癌中的表达率高达95.65%，在正常结直肠组织中呈阴性，在炎性增生组织中的表达率为16.12%（P<0.05）。Lgr5在管状腺瘤中的表达率为86.84%，在绒毛状息肉中的表达率为93.94%，两者与结肠癌中的表达率相当（P>0.05）。相关性分析表明，CD44和Lgr5的表达与肠息肉肿瘤发生进展呈正相关（rs=0.69377，P<0.0001；rs=0.81637，P<0.0001）。
Lgr5和CD44在结直肠癌组织中高表达，与临床病理特征密切相关。Lgr5和CD44的表达谱是区分结直肠癌与正常肠黏膜的显著特征。Lgr5比CD44与息肉肿瘤进展的相关性更强。这意味着在临床诊断早期结直肠疾病如息肉及其癌变患者时，联合检测Lgr5和CD44的表达具有重要意义且很有必要。
