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表达一种皮蝇抗菌肽编码序列的转基因甜橙植株显示减轻了柑橘溃疡病的症状。

Transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.

机构信息

Laboratorio de Agrobiotecnología, Departamento de Fisiología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina.

出版信息

J Biotechnol. 2013 Sep 20;167(4):412-9. doi: 10.1016/j.jbiotec.2013.07.019. Epub 2013 Jul 26.

Abstract

Citrus canker provoked by Xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. Xanthomonas infection is considered as an endemic disease in Northeast and Northwest Argentina, affecting as much as 10% of commercial citrus plantations. There is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. To introduce resistance to this disease, plants of Pineapple sweet orange were transformed with a genetic construct allowing constitutive accumulation of dermaseptin. In comparison with non-transformed plants, transgenic plants showed symptom reduction levels of up to 50% in in planta assays performed under controlled conditions.

摘要

由柑橘溃疡病菌(Xanthomonas axonopodis pv. citri)引起的柑橘溃疡病是一种细菌性疾病,在世界所有柑橘产区都造成了严重损失。在阿根廷东北部和西北部,溃疡病被认为是一种地方病,影响到多达 10%的商业柑橘种植园。目前,无论是在甜橙品种中,还是在用于商业品种嫁接的砧木中,都没有发现天然的抗性。为了引入对这种疾病的抗性,利用一个允许组成型积累 Dermaseptin 的遗传构建体转化了菠萝甜橙植株。与非转化植株相比,在受控条件下进行的体内试验中,转基因植株的症状减少水平高达 50%。

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