Sendin Lorena Noelia, Filippone María Paula
Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), EEAOC-CONICET, Av. William Cross 3150, Las Talitas, T4101XAC, Tucumán, Argentina.
Methods Mol Biol. 2019;1864:179-190. doi: 10.1007/978-1-4939-8778-8_13.
Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to the classical breeding approach is the use of genetic transformation, which provides the means for adding a single agronomic trait to a plant without otherwise altering its phenotype. Agrobacterium tumefaciens-mediated transformation has been carried out with numerous hybrids and citrus species. This technique allowed us to introduce the Bs2 gene in Citrus, as well as to increase citrus canker resistance in transgenic Bs2 gene-expressing lines.
培育抗病性是任何植物育种计划的最重要组成部分之一。柑橘传统育种方法（芽变选种、杂交育种和其他育种途径）是一项艰巨的任务，并且常常受到幼年期长、高度杂合性、多胚性、自交不亲和性以及生殖器官败育的阻碍。经典育种方法的一种有趣替代方法是使用遗传转化，它为在不改变植物表型的情况下向植物添加单一农艺性状提供了手段。根癌农杆菌介导的转化已在众多柑橘杂种和品种中进行。这项技术使我们能够将Bs2基因导入柑橘，并提高表达转基因Bs2基因的品系对柑橘溃疡病的抗性。
