Department of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, Bologna, Italy.
Bioresour Technol. 2013 Sep;144:529-38. doi: 10.1016/j.biortech.2013.07.006. Epub 2013 Jul 8.
A kinetic study of butane uptake and trichloroethylene (TCE) aerobic cometabolism was conducted by two suspended-cell (15 and 30°C) and two attached-cell (15 and 30°C) consortia obtained from the indigenous biomass of a TCE-contaminated aquifer. The shift from suspended to attached cells resulted in an increase of butane (15 and 30°C) and TCE (15°C) biodegradation rates, and a significant decrease of butane inhibition on TCE biodegradation. The TCE 15°C maximum specific biodegradation rate was equal to 0.011 mg(TCE ) mg(protein)(-1) d(-1) with suspended cells and 0.021 mg(TCE) mg(protein)(-1) d(-1) with attached cells. The type of mutual butane/TCE inhibition depended on temperature and biomass conditions. On the basis of a continuous-flow simulation, a packed-bed PFR inoculated with the 15 or 30°C attached-cell consortium could attain a 99.96% conversion of the studied site's average TCE concentration with a 0.4-0.5-day hydraulic residence time, with a low effect of temperature on the TCE degradation performances.
采用两种悬浮细胞（15 和 30°C）和两种附着细胞（15 和 30°C）生物膜，对丁烷吸收和三氯乙烯（TCE）好氧共代谢进行了动力学研究，这些生物膜取自受 TCE 污染含水层的土著生物量。从悬浮细胞到附着细胞的转变导致丁烷（15 和 30°C）和 TCE（15°C）生物降解率增加，而丁烷对 TCE 生物降解的抑制作用显著降低。15°C 时，TCE 的最大比生物降解速率分别为悬浮细胞时 0.011mg(TCE)mg(protein)(-1)d(-1)和附着细胞时 0.021mg(TCE)mg(protein)(-1)d(-1)。丁烷/TCE 相互抑制的类型取决于温度和生物量条件。基于连续流模拟，用 15 或 30°C 附着细胞生物膜接种的填充床 PFR 可以在 0.4-0.5 天的水力停留时间内将研究地点的 TCE 平均浓度转化 99.96%，且温度对 TCE 降解性能的影响较小。
