Santos Emanuel Burck dos, Koff Walter José, Grezzana Filho Tomaz de Jesus Maria, De Rossi Samanta Daiana, Treis Lisiane, Bona Silvia Regina, Pêgas Karla Laís, Katz Betina, Meyer Fabíola Schons, Marroni Norma Anair Possa, Corso Carlos Otávio
Division of Urology, Porto Alegre Clinics Hospital, RS, Brazil.
Acta Cir Bras. 2013 Aug;28(8):568-73. doi: 10.1590/s0102-86502013000800003.
To design an animal model of ischemia-reperfusion (I/R) in kidneys and evaluate the role that predetermined ranges of local hypothermia plays on markers of stress-oxydative as well as on histologic sections.
Twenty eight male rats Wistar, under general anesthesia, undergone right nephrectomy (G0, control group) followed by left kidney ischemia during 40 min. Four temperatures groups were designed, with seven animals randomized for each group: normothermic (G1, ±37ºC), mild hypothermia (G2, 26ºC), moderate hypothermia (G3, 15ºC) and deep hypothermia (G4, 4ºC). Left kidney temperature was assessed with an intraparenchymal probe. Left nephrectomy was performed after 240 min of reperfusion. After I/R a blood sample was obtained for f2-IP. Half of each kidney was sent to pathological evaluation and half to analyze CAT, SOD, TBARS, NO3, NO2.
Histopathology showed that all kidneys under I/R were significantly more injured than the G0 (p<0.001). TBARS had increased levels in all I/R groups compared with the G0 (p<0.001). CAT had a significant difference (p<0.03) between G1 and G4. Finally, no difference was found on SOD, NO3, NO2 nor on f2-IP.
This model of I/R was efficient to produce oxidative-stress in the kidney, showing that 4ºC offered significant decrease in free radicals production, although tissue protection was not observed.
设计一种肾脏缺血再灌注（I/R）动物模型，评估预定范围的局部低温对氧化应激标志物以及组织切片的作用。
28只雄性Wistar大鼠，在全身麻醉下，先进行右肾切除术（G0，对照组），随后左肾缺血40分钟。设计了四个温度组，每组随机分配7只动物：正常体温组（G1，±37℃）、轻度低温组（G2，26℃）、中度低温组（G3，15℃）和深度低温组（G4，4℃）。用实质内探头评估左肾温度。再灌注240分钟后进行左肾切除术。I/R后采集血样检测f2-IP。每个肾脏的一半送去进行病理评估，另一半用于分析CAT、SOD、TBARS、NO3、NO2。
组织病理学显示，所有I/R组的肾脏损伤均明显重于G0组（p<0.001）。与G0组相比，所有I/R组的TBARS水平均升高（p<0.001）。G1组和G4组之间CAT有显著差异（p<0.03）。最后，SOD、NO3、NO2以及f2-IP均未发现差异。
这种I/R模型能有效在肾脏产生氧化应激，表明4℃可显著降低自由基生成，尽管未观察到组织保护作用。
