Rolim Filho Epitácio Leite, Larrazabal Marcelo Correia de Araújo, Costa Lauri Ferreira da, Santos Saulo Monteiro dos, Santos Ricardo Monteiro dos, Aguiar José Lamartine de Andrade
Postgraduate Program in Surgery, Federal University of Pernambuco, Recife, PE, Brazil.
Acta Cir Bras. 2013 Aug;28(8):574-81. doi: 10.1590/s0102-86502013000800004.
To investigate by histomorphometry the distraction osteogenesis by Ilizarov technique in dog radius with the use of autologous stem cells in regenerated bone.
Ten dogs (20 radiuses) underwent the osteotomy of 20% of extension of their radiuses, and osteogenic distraction using the Ilizarov technique after this procedure at rate of 1mm per day divided into 0.5mm every 12 hours. The elongation was performed until the regeneration reached 20% of the total length of the radius. The stem cells were isolated, concentrated and injected in the regenerated bone, when it reached 10% of the length of the entire radius. The regenerated bone was evaluated using histomorphometric analysis when the elongation was 20% the size of radius.
The bone formation was evidenced by histomorphometric indices were significantly greater in the study group. In the histology evaluation the type of healing was mixed in 80% (intra membrane and endocondral) in both groups; the osteoblastic activity from moderate to intense was greater in the study group; and the space occupied by the newly-formed bone tissue was more evident in the study group.
The histomorphometric indices in this study expressing the microarchitecture, trabecular thickness, trabecular spacing, number of trabecula and quantity of bone that was significant in the group study. These data suggest that the use of undifferentiated stem cells autologous bone marrow in the regenerate bone induces osteogenesis and bone quality.
通过组织形态计量学研究在犬桡骨中使用Ilizarov技术进行牵张成骨并在再生骨中使用自体干细胞的情况。
10只犬（20根桡骨）接受桡骨20%长度的截骨术，术后采用Ilizarov技术以每天1毫米的速度进行成骨牵张，每12小时牵张0.5毫米。牵张直至再生达到桡骨总长度的20%。当再生骨达到整个桡骨长度的10%时，分离、浓缩干细胞并注入再生骨中。当牵张至桡骨大小的20%时，使用组织形态计量学分析评估再生骨。
组织形态计量学指标显示，研究组的骨形成明显更多。在组织学评估中，两组80%的愈合类型为混合性（膜内和软骨内）；研究组中从中度到强烈的成骨细胞活性更高；研究组中新形成的骨组织所占空间更明显。
本研究中的组织形态计量学指标，包括微观结构、小梁厚度、小梁间距、小梁数量和骨量，在研究组中具有显著意义。这些数据表明，在再生骨中使用未分化的自体骨髓干细胞可诱导成骨并改善骨质量。
