Asano Satoru, Takemura Tamiko, Katoh Keiichi, Taneda Masuzoh, Kitagawa Masanobu
Department of Intensive Care Unit, Japanese Red Cross Medical Center.
J Med Dent Sci. 2011 Dec 28;58(4):113-21.
This study was conducted to characterize the distribution of epithelial regeneration in lungs with diffuse alveolar damage (DAD) and to ascertain whether epithelial regeneration is associated with the underlying disease and/or DAD stage.
Lung specimens obtained at autopsy from 18 patients were studied using p63, PE-10 and Ki-67 immunostaining. The patients were categorized into two groups: patients with acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF; IPF group) and patients without IPF (non-IPF group).
p63-positive cells were mostly observed in the alveolar ducts. No significant difference in the appearance of the p63-positive cells was observed between the IPF and non-IPF groups or between the exudative and the proliferative stages. PE-10-positive cells were observed in the alveoli. The number of PE-10-positive cells was significantly higher during the proliferative stage than during the exudative stage. However, p63-positive cells and PE-10-positive cells appeared at day 6 in the earliest case during the exudative stage and Ki-67-positive cells were observed during the exudative stage.
Epithelial regeneration was not associated with the underlying disease. This study revealed that regenerative epithelial cells appeared at day 6 during the exudative stage and that the number of type Ⅱ pneumocytes increased significantly during the proliferative stage.
本研究旨在描述弥漫性肺泡损伤（DAD）肺上皮再生的分布情况，并确定上皮再生是否与潜在疾病和/或DAD分期相关。
对18例尸检获得的肺标本进行p63、PE-10和Ki-67免疫染色研究。患者分为两组：特发性肺纤维化急性加重患者（AE-IPF；IPF组）和无IPF患者（非IPF组）。
p63阳性细胞主要见于肺泡管。IPF组和非IPF组之间或渗出期和增殖期之间，p63阳性细胞的表现无显著差异。PE-10阳性细胞见于肺泡。增殖期PE-10阳性细胞数量显著高于渗出期。然而，在渗出期最早的病例中，p63阳性细胞和PE-10阳性细胞在第6天出现，且在渗出期观察到Ki-67阳性细胞。
上皮再生与潜在疾病无关。本研究表明，再生上皮细胞在渗出期第6天出现，且增殖期Ⅱ型肺泡上皮细胞数量显著增加。
