Biochemistry Department, University Hospital of South Manchester, Manchester, UK.
Ann Clin Biochem. 2013 Sep;50(Pt 5):489-91. doi: 10.1177/0004563213480758. Epub 2013 Jul 29.
Supported liquid extraction (SLE) techniques are relatively new compared to other sample preparation approaches such as solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PPE). We investigated the use of SLE as an alternative to SPE for the liquid chromatography tandem mass spectrometry (LC-MS/MS) measurement of aldosterone.
Samples (n = 83) were analysed by the routine method using SPE. The same samples were subsequently analysed using two different SLE 96-well plate devices (Thermo and Biotage) with methyl-tertiary butyl ether extraction. A direct comparison of the three extraction techniques on two different mass spectrometers was also performed.
Both results using SLE plates gave excellent agreement with the results from the SPE analysis. The area counts obtained with the Biotage plates were considerably higher than those obtained using the Thermo plate.
SLE is an acceptable alternative to SPE for the LC-MS/MS analysis of aldosterone. Using SLE reduces the time required for sample preparation.
与固相萃取（SPE）、液液萃取（LLE）和蛋白质沉淀（PPE）等其他样品制备方法相比，支持液体萃取（SLE）技术相对较新。我们研究了 SLE 作为 SPE 的替代方法，用于液相色谱串联质谱（LC-MS/MS）测量醛固酮。
采用 SPE 对 83 个样本进行常规方法分析。然后使用两种不同的 96 孔板 SLE 设备（Thermo 和 Biotage），用甲基叔丁基醚萃取，对同一样本进行分析。还在两台不同的质谱仪上对三种萃取技术进行了直接比较。
使用 SLE 板获得的两个结果与 SPE 分析结果非常吻合。Biotage 板获得的面积计数明显高于 Thermo 板。
SLE 是 LC-MS/MS 分析醛固酮的 SPE 替代方法。使用 SLE 减少了样品制备所需的时间。
