Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan 2nd Road, Guangzhou, 510080, China,
Calcif Tissue Int. 2013 Nov;93(5):481-6. doi: 10.1007/s00223-013-9765-z. Epub 2013 Jul 30.
This study compared the effects of alendronate (ALN) and strontium ranelate (SR) on bone mineral density (BMD), bone histomorphometry, and biomechanics in ovariectomized (OVX) rats. We randomly assigned 48 3-month-old female Sprague-Dawley rats to four groups: sham, OVX, ALN, and SR. Rats in the OVX, ALN, and SR groups received bilateral OVX. Rats in the ALN and SR groups were orally administrated ALN (7 mg/kg/week) and SR (500 mg/kg/day). Rats in the sham and OVX groups were treated with saline. All treatments continued for 12 weeks. Femoral BMD examination, distal femoral bone histomorphometry analysis, and biomechanical tests at the femoral diaphysis and metaphysis were performed to evaluate the effects of treatments in OVX rats. Results showed that both ALN and SR significantly increased femoral BMD (total femur, diaphyseal BMD, and distal metaphyseal BMD), distal femoral bone histomorphometric parameters (BV/TV, Tb.N, and Tb.Th), and femoral biomechanical parameters (maximum load, failure load, stiffness) compared with the OVX group (P < 0.05). No differences were found between ALN and SR in increasing femoral BMD, distal femoral bone histomorphometric parameters (BV/TV, Tb.N, and Tb.Th), and femoral diaphysis biomechanical parameters (maximum load, failure load, stiffness) (P > 0.05). The SR group was inferior to the ALN group in femoral metaphysis biomechanical parameters (P < 0.05). In conclusion, ALN (7 mg/kg/week) and SR (500 mg/kg/day) have similar effects by increasing BMD, distal femoral bone histomorphometric parameters, and femoral metaphysis biomechanical properties. Although ALN has greater effects than SR on distal femoral metaphysis biomechanical properties, in general, ALN and SR have comparable effects on the femur in OVX rats.
本研究比较了阿仑膦酸钠（ALN）和雷奈酸锶（SR）对去卵巢（OVX）大鼠骨密度（BMD）、骨组织形态计量学和生物力学的影响。我们将 48 只 3 月龄雌性 Sprague-Dawley 大鼠随机分为 4 组：假手术组、OVX 组、ALN 组和 SR 组。OVX 组、ALN 组和 SR 组大鼠行双侧卵巢切除术。ALN 组和 SR 组大鼠给予阿仑膦酸钠（7mg/kg/周）和雷奈酸锶（500mg/kg/天）灌胃。假手术组和 OVX 组大鼠给予生理盐水。所有治疗均持续 12 周。对股骨 BMD 检查、股骨远端骨组织形态计量学分析和股骨骨干及干骺端生物力学测试，以评估治疗对 OVX 大鼠的影响。结果显示，与 OVX 组相比，ALN 和 SR 均显著增加股骨 BMD（全股骨、骨干 BMD 和远端干骺端 BMD）、股骨远端骨组织形态计量学参数（BV/TV、Tb.N 和 Tb.Th）和股骨生物力学参数（最大载荷、断裂载荷、刚度）（P<0.05）。ALN 和 SR 对增加股骨 BMD、股骨远端骨组织形态计量学参数（BV/TV、Tb.N 和 Tb.Th）和股骨骨干生物力学参数（最大载荷、断裂载荷、刚度）无差异（P>0.05）。SR 组在股骨干骺端生物力学参数上劣于 ALN 组（P<0.05）。结论：ALN（7mg/kg/周）和 SR（500mg/kg/天）通过增加 BMD、股骨远端骨组织形态计量学参数和股骨干骺端生物力学特性，产生相似的效果。尽管 ALN 对股骨远端干骺端生物力学特性的影响大于 SR，但总体而言，ALN 和 SR 对 OVX 大鼠股骨的作用相当。
