应用胃液胃蛋白酶原和黑色素瘤相关基因 RNA 检测胃癌。
Gastric cancer detection using gastric juice pepsinogen and melanoma-associated gene RNA.
机构信息
Department of Surgery, School of Medicine, Catholic University of Daegu, Daegu, South Korea.
出版信息
Am J Clin Pathol. 2013 Aug;140(2):209-14. doi: 10.1309/AJCPOHXRM5IYXVOC.
OBJECTIVES
To develop a new method for gastric cancer detection with gastric juice using melanoma-associated gene (MAGE) RNA and pepsinogen (PG).
METHODS
In total, 183 gastric juice and paired serum specimens were obtained from 134 patients with gastric cancer and 49 healthy individuals. The gastric juice specimens were analyzed with MAGE A1 to A6 nested reverse transcription-polymerase chain reaction. The serum and gastric juice PG were measured with a PG I and II immunoassay.
RESULTS
The gastric juice PG I and PG I/II ratios were more accurate than those of serum. The combination test using the gastric PG I/II ratio and MAGE was the most accurate, with a sensitivity of 77.6% and a specificity of 87.8%. The sensitivity was 78.8% for stage I gastric cancer and not influenced by cancer location or pathologic type.
CONCLUSIONS
The combination test is potentially an additional tool for gastric cancer detection.
目的
利用黑色素瘤相关基因（MAGE）RNA 和胃蛋白酶原（PG）开发一种新的胃液胃癌检测方法。
方法
共收集 134 例胃癌患者和 49 例健康个体的 183 份胃液和配对血清标本。采用 MAGE A1 至 A6 巢式逆转录聚合酶链反应分析胃液标本。采用 PG I 和 II 免疫测定法测定血清和胃液 PG。
结果
与血清相比，胃液 PG I 和 PG I/II 比值更准确。使用胃 PG I/II 比值和 MAGE 的联合检测最为准确，其敏感性为 77.6%，特异性为 87.8%。Ⅰ期胃癌的敏感性为 78.8%，不受癌症位置或病理类型的影响。
结论
联合检测可能是胃癌检测的一种附加工具。
Zotero 插件