Chen Xianzhen, Xue Fei, Xie Tianhao, Luo Chun
Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, No. 415 FengYang Road, Shanghai, 200003, China.
Tumour Biol. 2013 Oct;34(5):3071-6. doi: 10.1007/s13277-013-0873-1. Epub 2013 Jul 30.
Smad nuclear interacting protein 1 (SNIP1) gene encodes a protein that contains a conservative C-terminal forkhead-associated domain and functions as a transcriptional coactivator to regulate cell proliferation and cancer progression. This study aimed to investigate the clinical and biological significance of SNIP1 expression in pituitary adenoma. We analyzed SNIP1 expressions in mouse fibroblast L929 cells and mouse pituitary adenoma AtT-20 cells by Western blotting. SNIP1 gene knockdown by small interfering RNA (siRNA) transfection was performed to evaluate SNIP1 function in pituitary adenoma cell lines. As expected, SNIP1 was found to be upregulated in pituitary adenoma cells. The mRNA and protein levels of SNIP1 were inhibited in AtT-20 cells transfected with siRNAs, which led to decreased proliferation, increased apoptosis, and cycle arrest of pituitary adenoma cells. Concomitantly, c-Myc and cyclin D1 protein levels were reduced. These findings may provide novel targets for the treatment of pituitary adenoma.
Smad核相互作用蛋白1（SNIP1）基因编码一种蛋白质，该蛋白质含有保守的C末端叉头相关结构域，并作为转录共激活因子发挥作用，以调节细胞增殖和癌症进展。本研究旨在探讨SNIP1表达在垂体腺瘤中的临床和生物学意义。我们通过蛋白质印迹法分析了小鼠成纤维细胞L929和小鼠垂体腺瘤AtT-20细胞中SNIP1的表达。通过小干扰RNA（siRNA）转染进行SNIP1基因敲低，以评估SNIP1在垂体腺瘤细胞系中的功能。正如预期的那样，发现SNIP1在垂体腺瘤细胞中上调。用siRNA转染的AtT-20细胞中SNIP1的mRNA和蛋白质水平受到抑制，这导致垂体腺瘤细胞增殖减少、凋亡增加和细胞周期停滞。同时，c-Myc和细胞周期蛋白D1的蛋白质水平降低。这些发现可能为垂体腺瘤的治疗提供新的靶点。
