Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Compr Physiol. 2013 Jul;3(3):1213-30. doi: 10.1002/cphy.c120021.
The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their disruption underlies a diverse group of heritable and acquired diseases that affect both pediatric and adult patients. Although experimental analyses used to study liver development and disease are typically performed in cell culture models or rodents, the zebrafish is increasingly used to complement discoveries made in these systems. Forward and reverse genetic analyses over the past two decades have shown that the molecular program for liver development is largely conserved between zebrafish and mammals, and that the zebrafish can be used to model heritable human liver disorders. Recent work has demonstrated that zebrafish can also be used to study the mechanistic basis of acquired liver diseases. Here, we provide a comprehensive summary of how the zebrafish has contributed to our understanding of human liver development and disease.
肝脏具有多种重要的合成和调节功能，这些功能在胎儿发育过程中获得，并贯穿生命始终。其功能紊乱是一组遗传性和获得性疾病的基础，这些疾病影响儿童和成人患者。尽管用于研究肝脏发育和疾病的实验分析通常在细胞培养模型或啮齿动物中进行，但斑马鱼越来越多地被用于补充这些系统中的发现。过去二十年的正向和反向遗传学分析表明，斑马鱼和哺乳动物的肝脏发育的分子程序在很大程度上是保守的，并且可以用斑马鱼来模拟遗传性人类肝脏疾病。最近的工作表明，斑马鱼也可用于研究获得性肝脏疾病的机制基础。在这里，我们全面总结了斑马鱼如何帮助我们理解人类肝脏发育和疾病。
