Hao Yalin, Jin Cheng, Wang Jiadong
Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Medical College of Shanghai Jiao Tong University,Shanghai, 200001, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 May;27(9):457-61.
To investigate the expression and clinical significance of Muc1, p63 protein in diffuse sclerosing variant of papillary thyroid carcinoma and conventional papillary thyroid carcinoma.
Immunohistochemistry (SP) was used to detect the expressions of Muc1, p63 protein in 30 samples of DSVPTC (experiment group) and 30 samples of CPTC (control group). Patients in two groups were matched in age, gender, tumor side, tumor size and date of diagnosis.
(1) The positive rate of Muc1 in DSVPTC and CPTC was 76.67% (23/30) and 53.33% (16/30) respectively, immunohistochemical staining expressed as brown or tan particles in the membrane or the cytoplasm,with a significant difference between the two groups (P < 0.05). The positive rate of p63 in DSVPTC and CPTC was 80% (24/30) and 43.33% (13/30) respectively, immunohistochemical staining expressed as a brown or tan particles in the muclei,with a significant difference between the two groups (P < 0.05). (2) Cervical lymph node metastasis rate in DSVPTC and CPTC was 50% (15/30) and 20% (6/30) respectively, with a significant difference between the two groups (P < 0.05). (3) In All cases,the positive rate of Muc1 in cervical lymph node metastasis group (21 cases) and without metastasis group (39 cases) was 85.71% (18/21) and 53.85% (21/39) respectively,with a significant difference between the two groups (P < 0.05); the positive rate of p63 was 95.24% (20/21) and 43.59% (17/39) respectively,with a significant difference between the two groups (P < 0.01). (4) Spearman rank correlation analysis showed that expression of Muc1 and p63 were positively correlated in both groups(r = 0.530,0. 386, P < 0.05).
(1) There are high expression of Muc1 and p63 protein in DSVPTC, and relatively low expression in CPTC, DSVPTC have a higher rate of cervical lymph node metastasis at the time of being diagnosed, compared to the CPTC. These results show that DSVPTC is a more biologically aggressive variant of the PTC. (2) Abnormal expression of Muc1 and p63 may be important to promote the progression and metastasis of PTC, thus they can be used as predictors of malignant behavior in PTC. (3) Muc1 and p63 may be synergistically promote proliferation and invasion metastasis of the PTC malignant cell.
探讨Muc1、p63蛋白在甲状腺乳头状癌弥漫硬化型（DSVPTC）及传统甲状腺乳头状癌（CPTC）中的表达及临床意义。
采用免疫组织化学（SP）法检测30例DSVPTC标本（实验组）及30例CPTC标本（对照组）中Muc1、p63蛋白的表达。两组患者在年龄、性别、肿瘤部位、肿瘤大小及诊断日期方面相匹配。
（1）DSVPTC和CPTC中Muc1的阳性率分别为76.67%（23/30）和53.33%（16/30），免疫组化染色表现为细胞膜或细胞质内的棕色或棕褐色颗粒，两组间差异有统计学意义（P<0.05）。DSVPTC和CPTC中p63的阳性率分别为80%（24/30）和43.33%（13/30），免疫组化染色表现为细胞核内的棕色或棕褐色颗粒，两组间差异有统计学意义（P<0.05）。（2）DSVPTC和CPTC的颈部淋巴结转移率分别为50%（15/30）和20%（6/30），两组间差异有统计学意义（P<0.05）。（3）所有病例中，颈部淋巴结转移组（21例）和无转移组（39例）中Muc1的阳性率分别为85.71%（18/21）和53.85%（21/39），两组间差异有统计学意义（P<0.05）；p63的阳性率分别为95.24%（20/21）和43.59%（17/39），两组间差异有统计学意义（P<0.01）。（4）Spearman等级相关分析显示，两组中Muc1和p63的表达均呈正相关（r=0.530，0.386，P<0.05）。
（1）DSVPTC中Muc1和p63蛋白表达较高，CPTC中表达相对较低，与CPTC相比，DSVPTC在诊断时颈部淋巴结转移率更高。这些结果表明DSVPTC是PTC中生物学行为更具侵袭性的变异型。（2）Muc1和p63的异常表达可能对PTC的进展和转移起重要作用，因此可作为PTC恶性行为的预测指标。（3）Muc1和p63可能协同促进PTC恶性细胞的增殖和侵袭转移。
