Michaels A, Herrin D L
Biology Department, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biochem Biophys Res Commun. 1990 Aug 16;170(3):1082-8. doi: 10.1016/0006-291x(90)90503-f.
In Chlamydomonas reinhardtii growing synchronously under a light-dark cycle, the major chloroplast mRNAs are constitutively present but are translated only during the light period. We show that translation of these mRNAs can be induced during the normal dark period by light or by acetate and the induction is blocked by an inhibitor of ATP synthesis, CCCP. Moreover, ATP levels in synchronous cells were found to be 2-5-fold lower during the dark than in the light period; the administration of acetate or light at the mid-dark period increased the ATP level 2-3-fold. These results exclude cell-cycle mediated control and suggest that the regulation of chloroplast translation in light-dark grown Chlamydomonas is mediated, at least in part, by ATP levels.
在明暗周期下同步生长的莱茵衣藻中，主要的叶绿体mRNA持续存在，但仅在光照期进行翻译。我们发现，在正常黑暗期，这些mRNA的翻译可被光或乙酸盐诱导，且这种诱导被ATP合成抑制剂CCCP阻断。此外，同步细胞中的ATP水平在黑暗期比光照期低2至5倍；在黑暗中期给予乙酸盐或光照可使ATP水平提高2至3倍。这些结果排除了细胞周期介导的控制，并表明在明暗生长的莱茵衣藻中，叶绿体翻译的调控至少部分是由ATP水平介导的。
