Hemmasi B
J Chromatogr. 1975 Feb 12;104(2):367-72. doi: 10.1016/s0021-9673(00)91860-0.
A ligand-exchange chromatographic proceudre for the selective separation of amino acids from inorganic ions is presented. It was found that the binding of amino acids to the nickel-Chelex 100 resin is pH dependent. At pH 8.5-9.1, only the basic amino acids lysine, histidine and arginine are quantitatively attached to the complex, whereas at pH 11, other amino acids with the exception of aspartic acid and glutamic acid are also bound, although not quantitatively. All of the amino acids can be eluted from the complex with 3 M ammonia solution without the displacement of nickel ions from the complex. This method can be used for the removal of the basic amino acids from solutions in the presence of inorganic ions as well as other amino acids.
本文介绍了一种用于从无机离子中选择性分离氨基酸的配体交换色谱方法。发现氨基酸与镍-螯合树脂100的结合依赖于pH值。在pH 8.5-9.1时，只有碱性氨基酸赖氨酸、组氨酸和精氨酸定量地附着在络合物上，而在pH 11时，除天冬氨酸和谷氨酸外的其他氨基酸也会结合，尽管不是定量的。所有氨基酸都可以用3M氨溶液从络合物中洗脱出来，而不会使镍离子从络合物中被置换。该方法可用于在存在无机离子以及其他氨基酸的情况下从溶液中去除碱性氨基酸。
