Takeo S, Tanonaka K, Hirai K, Kawaguchi K, Ogawa M, Yagi A, Fujimoto K
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
Biochem Pharmacol. 1990 Sep 1;40(5):1137-43. doi: 10.1016/0006-2952(90)90504-e.
The present study was undertaken to elucidate the possible effects of tanshinone VI, one of the extracts from the root of Salvia, on post-hypoxic recovery of cardiac contractile force. For this purpose, rat hearts were perfused for 45 min under reoxygenated conditions following 20-min hypoxic perfusion, and changes in tissue high-energy phosphates and calcium contents, and release of ATP metabolites and creatine kinase were examined. Post-hypoxic recovery of cardiac contractile force was augmented when hearts were treated with 42 nM tanshinone VI during hypoxia. This beneficial recovery was accompanied by enhanced restoration of myocardial high-energy phosphates, depression of hypoxia- and reoxygenation-induced increase in tissue calcium content, and suppression of release of ATP metabolites such as adenosine, inosine and hypoxanthine from the perfused heart. The results suggest that tanshinone VI is beneficial for the recovery of cardiac contractility after a certain period of oxygen-deficiency, possibly through mechanisms involving improvement of myocardial energy production upon oxygen-replenishment and/or inhibition of calcium accumulation in the cardiac cell.
本研究旨在阐明丹参根提取物之一的丹参酮VI对缺氧后心脏收缩力恢复的可能影响。为此，大鼠心脏在进行20分钟缺氧灌注后，再在复氧条件下灌注45分钟，检测组织高能磷酸盐和钙含量的变化以及ATP代谢产物和肌酸激酶的释放。当心脏在缺氧期间用42 nM丹参酮VI处理时，缺氧后心脏收缩力的恢复增强。这种有益的恢复伴随着心肌高能磷酸盐的恢复增强、缺氧和复氧诱导的组织钙含量增加的抑制以及灌注心脏中ATP代谢产物如腺苷、肌苷和次黄嘌呤释放的抑制。结果表明，丹参酮VI可能通过改善氧补充时的心肌能量产生和/或抑制心脏细胞中的钙积累机制，对一定时期缺氧后的心脏收缩力恢复有益。
