Hoffman W L, Jump A A, Smiley J D
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas.
Arthritis Rheum. 1990 Aug;33(8):1196-204. doi: 10.1002/art.1780330821.
Synovial tissue samples from 6 patients with rheumatoid arthritis were cultured, and the IgG antibodies isolated from the synovial culture supernatants were used to immunize rabbits to make 6 antiidiotypic (anti-Id) antibody preparations. After extensive adsorption, the rabbit anti-Id were tested in a solid-phase enzyme-linked immunosorbent assay (ELISA). Each anti-Id reacted predominantly with the immunizing synovial IgG and showed almost no reactivity with either pooled normal human serum IgG or with IgG from 50 normal donors. When identical amounts of matched rheumatoid arthritis serum IgG and synovial culture supernatant IgG were probed simultaneously with the corresponding rabbit anti-Id in an ELISA, 3 of 6 pairs demonstrated an increased concentration of specific idiotypes in the synovial culture supernatant IgG. Furthermore, when these 6 matched samples were subsequently analyzed by isoelectric focusing, individual IgG antibodies in 5 of 6 synovial IgG samples revealed enhanced reactivity with the corresponding rabbit anti-Id preparations, when compared with matched serum IgG. This increased synovial concentration of specific idiotypes detected by both the ELISA and isoelectric focusing was compatible with enhanced synovial tissue synthesis of the antibodies involved. These specific Id/anti-Id reactivities were not blocked by excess normal human Fc, Fab, or F(ab')2 fragments, indicating a lack of association of the stimulating synovial antibodies with rheumatoid factors or antibodies against other IgG fragments (pepsin agglutinators).
对6例类风湿性关节炎患者的滑膜组织样本进行培养，从滑膜培养上清液中分离出的IgG抗体用于免疫兔子，制备6种抗独特型（抗Id）抗体制剂。经过广泛吸附后，在固相酶联免疫吸附测定（ELISA）中检测兔抗Id。每种抗Id主要与免疫用的滑膜IgG反应，与正常人混合血清IgG或50名正常供体的IgG几乎无反应。当在ELISA中用相应的兔抗Id同时检测等量匹配的类风湿性关节炎血清IgG和滑膜培养上清液IgG时，6对中有3对显示滑膜培养上清液IgG中特异性独特型浓度增加。此外，当随后通过等电聚焦分析这6个匹配样本时，与匹配的血清IgG相比，6个滑膜IgG样本中有5个的单个IgG抗体与相应的兔抗Id制剂反应增强。通过ELISA和等电聚焦检测到的滑膜中特异性独特型浓度增加，与滑膜组织中相关抗体合成增强一致。这些特异性Id/抗Id反应性不被过量的正常人Fc、Fab或F(ab')2片段阻断，表明刺激性滑膜抗体与类风湿因子或抗其他IgG片段的抗体（胃蛋白酶凝聚素）缺乏关联。
