[双斑虻脂肪成分的提取及其气相色谱-质谱联用分析]
[Extraction of fatty constituents from Tabanus bivittatus and their analysis by GC-MS].
作者信息
Ding Cheng-Hua, Cao Feng-Pu, Wang Yan-Hua, Yang Ming-Li
机构信息
School of Chemistry and Pharmaceutical Engineering/School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China.
出版信息
Zhong Yao Cai. 2013 Feb;36(2):188-90.
OBJECTIVE
To optimize the extracting condition of fatty constituents from Tabanus bivittatus and analyse by GC-MS.
METHODS
Taking fatty yield as index, the extraction technology parameters of fatty constituents of Tabanus bivittatus ere optimized by single factor test and orthogonal test.
RESULTS
The optimum extraction parameters were as follows: the ratio of acetone to petroleum ether 1: 2, extraction time 2 h, solid-liquid ratio 1: 50. Twenty-one fatty acids were identified by GC-MS.
CONCLUSION
The process is reasonable and with good reproducibility. The main components of fatty acids in Tabanus bivittatus are palmitoleic acid, palmitic acid, linoleic acid, oleic acid and stearic acid.
目的
优化双斑黄虻脂肪成分的提取条件并采用气相色谱 - 质谱联用仪进行分析。
方法
以脂肪得率为指标，通过单因素试验和正交试验对双斑黄虻脂肪成分的提取工艺参数进行优化。
结果
最佳提取参数如下：丙酮与石油醚比例为1∶2，提取时间为2小时，固液比为1∶50。通过气相色谱 - 质谱联用仪鉴定出21种脂肪酸。
结论
该工艺合理，重现性良好。双斑黄虻脂肪酸的主要成分是棕榈油酸、棕榈酸、亚油酸、油酸和硬脂酸。
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