Lepri L, Desideri P G, Landini M, Tanturli G
J Chromatogr. 1975 Jun 18;109(2):365-76. doi: 10.1016/s0021-9673(01)91809-6.
The chromatographic characteristics of 58 phenols on Rexyn 102 and Dowex 50-X4 thin layers in both the acidic and sodium salt forms have been studied, using elution with water, water-alcohol mixtures and aqueous salt solutions at different pH values. The influence of the percentage of alcohol, the pH and the ionic strength on the chromatographic behaviour of these phenols was investigated. The validity of the relationships among the RF values, the pH of the eluent and the pKa of the phenol has been verified on Dowex 50-X4 (Na+) thin layers. It has been shown that from the RFac, RFalk and pKa values of the different phenols, it is possible to predict their behaviour over the whole pH range and therefore to select the best conditions for their chromatographic separation.
研究了58种酚类化合物在酸性和钠盐形式的Rexyn 102和Dowex 50-X4薄层上的色谱特性，采用不同pH值的水、水-醇混合物和盐水溶液进行洗脱。考察了醇的百分比、pH值和离子强度对这些酚类色谱行为的影响。在Dowex 50-X4（Na+）薄层上验证了比移值、洗脱液pH值和酚的pKa之间关系的有效性。结果表明，根据不同酚类的RFac、RFalk和pKa值，可以预测它们在整个pH范围内的行为，从而选择最佳的色谱分离条件。
