Maastricht University Medical Centre, Department of Medical Microbiology, P Debyelaan 25, 6229 HX Maastricht, The Netherlands.
Future Microbiol. 2013 Aug;8(8):1027-37. doi: 10.2217/fmb.13.61.
To investigate the prevalence and genetic background of Escherichia coli collected from different patient populations in the Euroregion Meuse-Rhine.
MATERIALS & METHODS: Susceptibility testing was performed on 1651 E. coli isolates with broth microdilution. Their genetic background was determined using pulsed-field gel electrophoresis and multilocus sequence typing.
The prevalence of resistance varied significantly between the populations. Approximately 10% of the E. coli isolates were multidrug-resistant and/or a β-lactamase producer. The most prevalent extended-spectrum β-lactamase type was CTX-M-15 and ST131 was the most prevalent multilocus sequence typing type. RESULTS from pulsed-field gel electrophoresis of the ST131 isolates indicate the spread of these isolates in the Euroregion.
E. coli ST131 was the most prevalent sequence type in our Euroregional study. It is essential to control the spread of these resistant strains (e.g., with infection-control policies, antibiotic stewardship programs and antibiotic resistance surveillance). In this way we could observe shifts in the prevalence of resistance of the E. coli population and act accordingly.
调查来自默兹-莱茵欧区不同患者人群的大肠杆菌的流行情况和遗传背景。
采用肉汤微量稀释法对 1651 株大肠杆菌分离株进行药敏试验。采用脉冲场凝胶电泳和多位点序列分型法确定其遗传背景。
不同人群中耐药率差异显著。约 10%的大肠杆菌分离株为多药耐药株和/或产β-内酰胺酶株。最常见的扩展型β-内酰胺酶类型为 CTX-M-15，最常见的多位点序列分型类型为 ST131。ST131 分离株的脉冲场凝胶电泳结果表明这些分离株在欧区的传播。
在我们的欧区研究中，大肠杆菌 ST131 是最常见的序列类型。控制这些耐药菌株的传播（例如，通过感染控制政策、抗生素管理计划和抗生素耐药性监测）至关重要。通过这种方式，我们可以观察到大肠杆菌种群耐药性的流行情况发生变化，并采取相应的措施。
