Section of Neurosurgery, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA.
Opt Lett. 2013 Aug 1;38(15):2786-8. doi: 10.1364/OL.38.002786.
We report an accurate, precise and sensitive method and system for quantitative fluorescence image-guided neurosurgery. With a low-noise, high-dynamic-range CMOS array, we perform rapid (integration times as low as 50 ms per wavelength) hyperspectral fluorescence and diffuse reflectance detection and apply a correction algorithm to compensate for the distorting effects of tissue absorption and scattering. Using this approach, we generated quantitative wide-field images of fluorescence in tissue-simulating phantoms for the fluorophore PpIX, having concentrations and optical absorption and scattering variations over clinically relevant ranges. The imaging system was tested in a rodent model of glioma, detecting quantitative levels down to 20 ng/ml. The resulting performance is a significant advance on existing wide-field quantitative imaging techniques, and provides performance comparable to a point-spectroscopy probe that has previously demonstrated significant potential for improved detection of malignant brain tumors during surgical resection.
我们报道了一种用于定量荧光图像引导神经外科的准确、精密且灵敏的方法和系统。利用低噪声、高动态范围 CMOS 阵列，我们实现了快速（每个波长的积分时间低至 50 毫秒）高光谱荧光和漫反射检测，并应用了校正算法来补偿组织吸收和散射的失真影响。使用这种方法，我们针对荧光团 PpIX 在组织模拟体模中生成了定量宽场荧光图像，其浓度和光学吸收以及散射变化涵盖了临床相关的范围。该成像系统在胶质瘤的啮齿动物模型中进行了测试，可检测到低至 20ng/ml 的定量水平。与之前已证明在手术切除过程中对恶性脑肿瘤的检测具有显著改善潜力的单点光谱探头相比，该系统的性能有了显著提高，是现有宽场定量成像技术的重大进展。
