Kabuto M, Hayashi M, Nakagawa T, Katsumura H, Hosotani K, Kobayashi H, Kubota T, Ishii Y, Ishimori Y
Department of Neurosurgery, Fukui Medical School, Japan.
No To Shinkei. 1990 Apr;42(4):339-43.
Small animal models such as the rat have serious limitations for multiple human scale instrumentation, surgical manipulations, and computerized tomographic (CT) evaluations, so that large animal models are required for the study using them. Although brain tumors induced with Rous sarcoma virus in neonatal beagle or adult monkey had been reported, these animals are very expensive ones for tumor research. A major drawback of virally induced brain tumor model is, moreover, the need for specialized viral facilities and safety precautions for laboratory personnel. In this paper, a cat glioma model implanted with C6 glioma cells derived from rats injected with N-nitrosomethylurea is reported. For an implantation dose of 5 x 10(5) cells/50 microliters, C6 glioma cells were suspended in modified Eagle medium supplemented with 10% fetal bovine serum and 0.5% agar. Twenty adult mongrel cats were injected with 5 x 10(5) C6 glioma cells intracerebrally. Implanted cats had brain tumors of about 10 mm in diameter with a yield of 80%. The mean survival was about 3 weeks after implantation. Tumors developed as spheroidal, hemorrhagic masses with central areas of necrosis and peripheral edema. They were located within the parenchyma of the implanted region. This tumor possessed many of the histological and radiological characteristics of human glioblastoma such as the following: Areas of hemorrhage and necrosis surrounded by pseudopallisading were observed within the tumor consisting of spindle-shaped cells with pleomorphic nuclei. A mass lesion with ring or garland-like enhancement surrounded by brain edema was shown on the CT scans.(ABSTRACT TRUNCATED AT 250 WORDS)
诸如大鼠之类的小动物模型在用于多种人体规模的仪器、外科手术操作以及计算机断层扫描（CT）评估方面存在严重局限性，因此在使用这些仪器、操作和评估的研究中需要大型动物模型。尽管已有报道称新生比格犬或成年猴子可被劳斯肉瘤病毒诱发脑肿瘤，但这些动物用于肿瘤研究的成本非常高。此外，病毒诱发脑肿瘤模型的一个主要缺点是需要专门的病毒设施以及针对实验室人员的安全预防措施。在本文中，报道了一种植入了源自用N - 亚硝基甲脲注射的大鼠的C6胶质瘤细胞的猫胶质瘤模型。对于5×10⁵个细胞/50微升的植入剂量，将C6胶质瘤细胞悬浮于补充有10%胎牛血清和0.5%琼脂的改良伊格尔培养基中。对20只成年杂种猫进行脑内注射5×10⁵个C6胶质瘤细胞。植入细胞的猫产生了直径约10毫米的脑肿瘤，成功率为80%。植入后平均存活时间约为3周。肿瘤发展为球形、出血性肿块，伴有中央坏死区和周边水肿。它们位于植入区域的实质内。这种肿瘤具有人类胶质母细胞瘤的许多组织学和放射学特征，如下：在由具有多形核的梭形细胞组成的肿瘤内观察到有假栅栏状环绕的出血和坏死区域。CT扫描显示有一个伴有脑水肿环绕的呈环形或花环样强化的肿块病变。（摘要截取自250词）
