An assessment of in ovo toxicity of the flame retardant 1,2-dibromo-4-(1,2-dibromoethyl) cyclohexane (TBECH) in the zebra finch.

Embryonic toxicity of a brominated flame retardant, TBECH (1,2-dibromo-4-(1, 2-dibromoethyl) cyclohexane) was investigated using the zebra finch (Taeniopygia guttata) as our model. Using in ovo dosing procedures, we injected the technical TBECH compound, consisting of 50:50 α-/β-isomers, directly into the yolk of freshly laid eggs at concentrations of 2.3-94 ng/g egg. No significant effects were observed in terms of growth or survival for either pre-hatch embryos or post-hatch chicks. Analysis of tissue samples at various developmental stages suggests that α-/β-TBECH was metabolized rapidly, and thus is unlikely to cause any direct, long-term effects on the development of zebra finch embryos or offspring.