Biotechnology Laboratory, Department of Food Science and Human Nutrition, Faculty of Agriculture and Veterinary Medicine, Al-Qassim University, Qassim, Kingdom of Saudi Arabia,
Appl Biochem Biotechnol. 2013 Oct;171(4):883-99. doi: 10.1007/s12010-013-0405-x. Epub 2013 Aug 1.
Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified organism varieties in samples were used. The positive results obtained from GMOScreen 35S and NOS were identified using specific primer pairs. The results indicated that all rice samples gave negative results for the presence of 35S and NOS terminator. About 26 % of samples containing soybean were positive for 35S and NOS terminator and 44 % of samples containing maize were positive for the presence of 35S and/or NOS terminator. The results showed that 20.4 % of samples was positive for maize line Bt176, 8.8 % was positive for maize line Bt11, 8.8 % was positive for maize line T25, 5.9 % was positive for maize line MON 810, and 5.9 % was positive for StarLink maize. Twelve samples were shown to contain <3 % of genetically modified (GM) soy and 6 samples >10 % of GM soy. Four samples containing GM maize were shown to contain >5 % of GM maize MON 810. Four samples containing GM maize were shown to contain >1 % of StarLink maize. Establishing strong regulations and certified laboratories to monitor GM foods or crops in Saudi market is recommended.
定性和定量的基于 DNA 的方法被应用于检测沙特阿拉伯市场样品中的转基因食品。2009 年和 2010 年,从 Al-Qassim、利雅得和 Mahdina 收集了 200 个样本。用于检测样品中转基因生物品种的 GMOScreen 35S 和 NOS 试剂盒被使用。从 GMOScreen 35S 和 NOS 获得的阳性结果使用特异性引物对进行鉴定。结果表明,所有大米样本均未检测到 35S 和 NOS 终止子的存在。约 26%的含大豆样本对 35S 和 NOS 终止子呈阳性,44%的含玉米样本对 35S 和/或 NOS 终止子呈阳性。结果显示,20.4%的样本对玉米品系 Bt176 呈阳性,8.8%的样本对玉米品系 Bt11 呈阳性,8.8%的样本对玉米品系 T25 呈阳性,5.9%的样本对玉米品系 MON 810 呈阳性,5.9%的样本对 StarLink 玉米呈阳性。12 个样本显示含有<3%的转基因(GM)大豆,6 个样本显示含有>10%的 GM 大豆。4 个含 GM 玉米的样本显示含有>5%的 GM 玉米 MON 810。4 个含 GM 玉米的样本显示含有>1%的 StarLink 玉米。建议在沙特市场建立强有力的监管和认证实验室,以监测转基因食品或作物。