Beheshti Zadeh Ramin, Safaeian Shila, Moslemi Elham, Mosavi Nadushen Rezvan, Esfahani Kasra
Department of Food Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
Iran J Pharm Res. 2022 Jun 14;21(1):e126921. doi: 10.5812/ijpr-126921. eCollection 2022 Dec.
Soybean and maize are the most cultivated genetically modified (GM) plants. Because of the increase in the imports of GM products to Iran, infant formula and baby food, which is consumed by babies during their first month of life, can also contain soybean and maize. It has become fundamental to screen these types of products.
The present study aimed to investigate the GM corn and soybean in baby food and infant formula using real-time polymerase chain reaction (PCR).
A total of 60 baby food and infant formulas were collected randomly from the local drugstores in Tehran. Genomic DNA was extracted from all samples, then by real-time PCR detection, tested Pat/NOS. Internal control genes zein and lectin were used for maize and soybean, respectively.
Results showed that 5% of infant formulas and 5% of baby food, two Iranian and one imported baby food, and two imported and one Iranian infant formula were positive for pat. However, NOS was detected in none of the samples. The results showed positive results for the presence of the pat gene in the products without an appropriate label.
This article provides evidence of GM maize and soybean presence in baby food and infant formula in Iran.
大豆和玉米是种植最为广泛的转基因作物。由于伊朗进口转基因产品的增加，婴儿配方奶粉和婴儿食品（婴儿出生后第一个月食用）也可能含有大豆和玉米。因此，对这类产品进行筛查至关重要。
本研究旨在通过实时聚合酶链反应（PCR）检测婴儿食品和婴儿配方奶粉中的转基因玉米和大豆。
从德黑兰当地药店随机收集了60份婴儿食品和婴儿配方奶粉。从所有样品中提取基因组DNA，然后通过实时PCR检测pat/NOS。分别使用内部对照基因玉米醇溶蛋白和凝集素检测玉米和大豆。
结果显示，5%的婴儿配方奶粉和5%的婴儿食品呈pat阳性，其中包括两种伊朗产和一种进口婴儿食品，以及两种进口和一种伊朗产婴儿配方奶粉。然而，所有样品中均未检测到NOS。结果表明，未加适当标签的产品中存在pat基因。
本文提供了伊朗婴儿食品和婴儿配方奶粉中存在转基因玉米和大豆的证据。
