微小 RNA-143 抑制人非小细胞肺癌的迁移和侵袭及其相关机制。

MicroRNA-143 inhibits migration and invasion of human non-small-cell lung cancer and its relative mechanism.

机构信息

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.

出版信息

Int J Biol Sci. 2013 Jul 18;9(7):680-92. doi: 10.7150/ijbs.6623. Print 2013.

BACKGROUND

MicroRNAs (miRNAs) play important roles in many biological processes, including cancer development. Among those miRNAs, miR-143 shows tumor-suppressive activity in some human cancers. However, the function and mechanism of miR-143 in lung cancer cells remains unknown. Here we explored the role of miR-143 in lung cancer.

RESULTS

According to qRT-PCR, we found that miR-143 was notably down-regulated in 19 NSCLC tissues and 5 cell lines. In vitro experiments showed us that miR-143 could significantly suppress the migration and invasion of NSCLC cell lines while it had no effects on the growth of NSCLC cell lines, and in vivo metastasis assay showed the same results. Finally, we found that the mechanism of miR-143 inhibiting the migration and invasion of NSCLC might be through targeting CD44v3.

CONCLUSIONS

The up-regulated miR-143 in lung cancer could significantly inhibit cell migration and invasion, and this might work through targeting CD44v3, which was newly identified by us.

背景

微小 RNA（miRNA）在许多生物学过程中发挥着重要作用，包括癌症的发生。在这些 miRNA 中，miR-143 在一些人类癌症中表现出肿瘤抑制活性。然而，miR-143 在肺癌细胞中的功能和机制尚不清楚。在这里，我们研究了 miR-143 在肺癌中的作用。

结果

根据 qRT-PCR，我们发现 miR-143 在 19 个 NSCLC 组织和 5 个细胞系中明显下调。体外实验表明，miR-143 可显著抑制 NSCLC 细胞系的迁移和侵袭，而对 NSCLC 细胞系的生长没有影响，体内转移实验也得到了相同的结果。最后，我们发现 miR-143 抑制 NSCLC 迁移和侵袭的机制可能是通过靶向 CD44v3。