Yao Cui-Ping, Wang Jing, Yang Yang, Dong Yan-Hua, Xue Yang, Mei Jian-Sheng, Zeng Wei-Hui, Zhang Zhen-Xi
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 May;33(5):1299-303.
Folate receptor (FR) is particularly upregulated in many epithelial cancer cells membrane and limited distribution is found in normal tissues. In the present work, the folic acid protected gold nanoparticles (FA-GNPs) were synthesized by a simple and quick method, in which chloroauric acid (HAuCl4) was reduced by sodium borohydride (NaBH4) in the presence of FA is used as stabilizer. UV-Visible spectroscopy and transmission electron microscopy (TEM) were used to characterize the shape and size distribution of the produced FA-GNPs. X-ray photoelectron spectroscopy and cell experiment were employed to confirm the immobilization of FA and GNPs. The results showed that FA-GNPs have a good size distribution in the 3-5 nm diameter range. Moreover, it is very stable even in solution with high concentration of salt (up to 3.5% NaCl), and even high speed centrifuges of 25 000 r x min(-1) could not cause aggregation. The nanoparticles could be used to detect cancer cells.
叶酸受体（FR）在许多上皮癌细胞膜中特别上调，而在正常组织中的分布有限。在本研究中，通过一种简单快速的方法合成了叶酸保护的金纳米颗粒（FA-GNPs），其中在作为稳定剂的FA存在下，用硼氢化钠（NaBH4）还原氯金酸（HAuCl4）。利用紫外可见光谱和透射电子显微镜（TEM）对所制备的FA-GNPs的形状和尺寸分布进行表征。采用X射线光电子能谱和细胞实验来确认FA和GNPs的固定化。结果表明，FA-GNPs在直径3-5nm范围内具有良好的尺寸分布。此外，即使在高浓度盐溶液（高达3.5%NaCl）中它也非常稳定，甚至25000r×min(-1)的高速离心机也不会导致聚集。这些纳米颗粒可用于检测癌细胞。
