Pratummo Kanita, Jetsrisuparb Arunee, Fucharoen Supan, Tripatara Amporn
Hematology. 2014 Apr;19(3):175-80. doi: 10.1179/1607845413Y.0000000110. Epub 2013 Nov 25.
Hepcidin is a key regulator of body iron homeostasis. The inflammatory cytokine interleukin (IL)-6 has been reported to upregulate expression of the hepcidin (HAMP) gene in monocytes. The purpose of this work was to determine HAMP expression at steady state in monocytes of splenectomized and non-splenectomized patients with HbE-β-thalassemia compared with normal controls.
Levels of HAMP mRNA were measured using real-time reverse transcriptase polymerase chain reaction. Plasma IL-6, soluble transferrin receptor (sTfR), and ferritin levels were determined by enzyme-linked immunosorbent assay, and C-reactive protein (CRP) by nephelometry.
Levels of HAMP mRNA, CRP, IL-6, sTfR, and ferritin were significantly higher in both groups of patients with thalassemia than controls, but were not different between splenectomized and non-splenectomized patients. Monocyte HAMP mRNA content of patients with thalassemia correlated with plasma IL-6 and CRP levels.
Patients with HbE-β-thalassemia have persistent elevation of the plasma inflammatory cytokines, CRP, and IL-6, and the latter could be responsible (in part) to the induction of HAMP expression in monocytes of patients with HbE-β-thalassemia.
铁调素是机体铁稳态的关键调节因子。据报道，炎性细胞因子白细胞介素（IL）-6可上调单核细胞中铁调素（HAMP）基因的表达。本研究旨在比较正常对照、脾切除和未脾切除的HbE-β地中海贫血患者单核细胞中HAMP在稳态时的表达情况。
采用实时逆转录聚合酶链反应检测HAMP mRNA水平。采用酶联免疫吸附测定法测定血浆IL-6、可溶性转铁蛋白受体（sTfR）和铁蛋白水平，采用散射比浊法测定C反应蛋白（CRP）水平。
两组地中海贫血患者的HAMP mRNA、CRP、IL-6、sTfR和铁蛋白水平均显著高于对照组，但脾切除和未脾切除患者之间无差异。地中海贫血患者单核细胞HAMP mRNA含量与血浆IL-6和CRP水平相关。
HbE-β地中海贫血患者血浆炎性细胞因子、CRP和IL-6持续升高，后者可能（部分）导致HbE-β地中海贫血患者单核细胞中HAMP表达上调。
