Gansu Provincial Key Lab of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, PR China.
Plant Physiol Biochem. 2013 Oct;71:96-102. doi: 10.1016/j.plaphy.2013.07.001. Epub 2013 Jul 13.
Podophyllotoxin (PDT) and its derivatives, which are isolated from the Podophyllum species, are widely used in the clinical setting. The present study was designed to analyze the correlation between PDT levels in the rhizomes of Podophyllum hexandrum (P. hexandrum) and Dysosma versipellis (D. versipellis) and the nutrients in soil. We also aimed to investigate the influence of Fe(2+) and Mn(2+) on the enzyme activity of phenylalanine ammonia lyase (PAL), cinnamyl alcohol-dehydrogenase (CAD), and deoxypodophyllotoxin 6-hydroylase (DOP6H) and PDT accumulation via P. hexandrum tissue culture. The results showed that PDT accumulation was positively correlated with the NO3(-), PO4(3-), Na(+), Fe, and Mn levels and was negatively correlated with the SO4(2-) and K(+) levels, while the correlation with the Mg(2+), Ca(2+), Cu and Zn levels was not significant. The Fe(2+) and Mn(2+) levels were associated with the increased activity of PAL and CAD at 3-18 days; Fe(2+) enhanced the activity levels by 2.66- and 1.76-fold, respectively, and Mn(2+) was associated with a 1.68- and 1.10-fold increase in activity levels, respectively, compared with the control (CK) at 18 days. DOP6H activity was enhanced by Mn(2+), but it was not significantly affected by Fe(2+). Finally, PDT production was enhanced approximately 60% and 34% by Fe(2+) and Mn(2+), respectively, compared with CK at 16 days. These observations may be useful for the generation of PDT and related lignans via commercial cultivation as well as cell and tissue culture of P. hexandrum and other related plant resources.
鬼臼毒素（PDT）及其衍生物是从鬼臼属植物中分离得到的，广泛应用于临床。本研究旨在分析滇藏鬼臼（P. hexandrum）和桃儿七（D. versipellis）根茎中 PDT 水平与土壤养分之间的相关性。我们还旨在研究 Fe(2+)和 Mn(2+)对苯丙氨酸解氨酶（PAL）、肉桂醇脱氢酶（CAD）和去氧鬼臼毒素 6-羟化酶（DOP6H）的酶活性以及通过滇藏鬼臼组织培养积累 PDT 的影响。结果表明，PDT 积累与 NO3(-)、PO4(3-)、Na(+)、Fe 和 Mn 水平呈正相关，与 SO4(2-)和 K(+)水平呈负相关，而与 Mg(2+)、Ca(2+)、Cu 和 Zn 水平的相关性不显著。Fe(2+)和 Mn(2+)水平与 PAL 和 CAD 在 3-18 天的活性增加有关；与对照（CK）相比，Fe(2+)分别使活性水平提高了 2.66 倍和 1.76 倍，Mn(2+)分别使活性水平提高了 1.68 倍和 1.10 倍，分别在第 18 天。Mn(2+)增强了 DOP6H 的活性，但 Fe(2+)对其影响不显著。最后，与 CK 相比，在第 16 天，Fe(2+)和 Mn(2+)分别使 PDT 产量提高了约 60%和 34%。这些观察结果可能有助于通过商业种植以及滇藏鬼臼和其他相关植物资源的细胞和组织培养来产生 PDT 和相关木脂素。
