孕酮通过受体介导的方式使 LPS 降低的小鼠外周血单个核细胞 FAAH 活性恢复。
Progesterone reverts LPS-reduced FAAH activity in murine peripheral blood mononuclear cells by a receptor-mediated fashion.
机构信息
Laboratory of Physiopathology of Pregnancy and Labor, Center for Pharmacological and Botanical Studies, National Research Council, School of Medicine, University of Buenos Aires, Argentina.
出版信息
Mol Cell Endocrinol. 2013 Dec 5;381(1-2):97-105. doi: 10.1016/j.mce.2013.07.020. Epub 2013 Jul 29.
Increased anandamide concentrations are associated with pregnancy failure. Anandamide levels are regulated by the fatty acid amide hydrolase (FAAH). The aim of the study was to investigate the role of progesterone (P) on FAAH modulation in murine peripheral blood mononuclear cells (PBMC) under septic conditions. We observed that in vivo administration of LPS to non-pregnant (NP) mice decreased FAAH activity of PBMC while in pregnant mice no changes in FAAH activity were observed. NP animals administered with P had a similar response to LPS as the pregnant animals. Also, NP mice injected with P antagonist and P showed that the effect of P on LPS-reduced FAAH activity was impaired. Furthermore, LPS produced a decrease in the ratio of PR-B/PR-A in NP animals. Our results showed that, in our model the endotoxin decreased PBMC's FAAH activity and this condition was reverted by P in a receptor-mediated fashion.
大麻素浓度的增加与妊娠失败有关。大麻素水平受脂肪酸酰胺水解酶（FAAH）调节。本研究旨在探讨孕激素（P）在脓毒症条件下对鼠外周血单个核细胞（PBMC）中 FAAH 调节的作用。我们观察到，内毒素脂多糖（LPS）给药于非妊娠（NP）小鼠会降低 PBMC 的 FAAH 活性，而在妊娠小鼠中 FAAH 活性没有变化。给予 P 的 NP 动物对 LPS 的反应与妊娠动物相似。此外，给予 P 拮抗剂和 P 的 NP 小鼠表明，P 对 LPS 降低 FAAH 活性的作用受损。此外，LPS 使 NP 动物的 PR-B/PR-A 比值降低。我们的结果表明，在我们的模型中，内毒素降低了 PBMC 的 FAAH 活性，而 P 以受体介导的方式逆转了这种情况。
Zotero 插件