Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Dev Dyn. 2013 Nov;242(11):1236-49. doi: 10.1002/dvdy.24020. Epub 2013 Sep 30.
The transient receptor potential melastatin (TRPM) gene family belongs to the superfamily of nonselective TRP ion channels. TRP channels are cellular sensors, detecting a multitude of inputs, including temperature, light, chemical, and mechanical stimuli. Recent studies revealed diverse roles during development, linking TRP channels to differentiation, proliferation, cell motility, cell death, and survival. A detailed description of this gene family in the zebrafish is still missing.
Phylogenetic analysis revealed 11 trpm genes in the zebrafish genome. The zebrafish orthologs of mammalian TRPM1 and TRPM4 are duplicated and quadruplicated, respectively, and TRPM8, a cold sensitive channel has been lost in zebrafish. Whole-mount in situ hybridization experiments revealed dynamic expression pattern of trpm genes in the developing embryo and early larva. Transcripts were mainly found in neural cell clusters, but also in tissues involved in ion homeostasis.
Our results suggest a role of TRPM channels in sensory information processing, including vision, olfaction, taste, and mechanosensation. An involvement in developmental processes is likely, as some trpm genes were found to be expressed in differentiating cells. Our data now provide a basis for functional analyses of this gene family of ion channels in the vertebrate model organism Danio rerio.
瞬时受体电位 melastatin (TRPM) 基因家族属于非选择性 TRP 离子通道超家族。TRP 通道是细胞传感器，可检测多种输入，包括温度、光、化学和机械刺激。最近的研究揭示了它们在发育过程中的多种作用，将 TRP 通道与分化、增殖、细胞迁移、细胞死亡和存活联系起来。TRPM 基因家族在斑马鱼中的详细描述仍然缺失。
系统发育分析显示斑马鱼基因组中有 11 个 trpm 基因。哺乳动物 TRPM1 和 TRPM4 的斑马鱼同源基因分别被复制和四倍化，而对冷敏感的 TRPM8 已在斑马鱼中丢失。全胚胎原位杂交实验显示 trpm 基因在发育胚胎和早期幼虫中的动态表达模式。转录本主要在神经细胞簇中发现，但也在参与离子稳态的组织中发现。
我们的结果表明 TRPM 通道在感觉信息处理中发挥作用，包括视觉、嗅觉、味觉和机械感觉。它们可能参与发育过程，因为一些 trpm 基因在分化细胞中表达。我们的数据现在为在脊椎动物模式生物斑马鱼中对该离子通道基因家族的功能分析提供了基础。
