丙二酸作为黄曲霉毒素生物合成的前体。

Malonate as a precursor in the biosynthesis of aflatoxins.

作者信息

Gupta S R, Prasanna H R, Viswanathan L, Venkitasurbramanian T A

出版信息

J Gen Microbiol. 1975 Jun;88(2):317-20. doi: 10.1099/00221287-88-2-317.

Abstract

Incorporation of [I-14C]acetate and [2-14C]malonate into aflatoxins by resting mycelia of Aspergillus parasiticus resuspended in different buffers was studied. A decrease in pH from 5-8 to 2-8, as well as addition of EDTA, markedly stimulated the incorporation of malonate but the effect on acetate incorporation was less pronounced. Mycelia took up comparatively more acetate than malonate, but more malonate (4-3%) entering mycelia was incorporated into aflatoxins than was acetate (1-6%). Furthermore, the addition of unlabelled acetate reduced the incorporation of label from [I-14C]acetate by 75% but from [2-14C]malonate by only 25%. These results suggest that malonate is an intermediate in aflatoxin synthesis and that is can be incorporated without prior conversion to acetate.

摘要

研究了将悬浮于不同缓冲液中的寄生曲霉静止菌丝体中[I-14C]乙酸盐和[2-14C]丙二酸盐掺入黄曲霉毒素的情况。pH从5.8降至2.8以及添加乙二胺四乙酸（EDTA）均显著刺激了丙二酸盐的掺入，但对乙酸盐掺入的影响则不太明显。菌丝体摄取的乙酸盐相对比丙二酸盐更多，但进入菌丝体的丙二酸盐（4.3%）比乙酸盐（1.6%）更多地掺入到黄曲霉毒素中。此外，添加未标记的乙酸盐可使[I-14C]乙酸盐的标记掺入减少75%，但[2-14C]丙二酸盐的标记掺入仅减少25%。这些结果表明丙二酸盐是黄曲霉毒素合成的中间体，并且它可以在无需预先转化为乙酸盐的情况下被掺入。