某些抑制剂对寄生曲霉静止菌丝体在合成培养基中产生黄曲霉毒素以及将乙酸-1-¹⁴C掺入黄曲霉毒素的影响。

Effect of some inhibitors on aflatoxin-production in a synthetic medium and on the incorporation of acetate-1- 14C into aflatoxins by resting mycelia of Aspergillus parasiticus.

作者信息

Gupta S R, Prasanna H R, Viswanathan L, Venkitasubramanian T A

出版信息

Bull Environ Contam Toxicol. 1976 Apr;15(4):447-53. doi: 10.1007/BF01685070.

Abstract

The effect of a number of metabolic inhibitors on the incorporation of acetate-1-14C into aflatoxins was investigated, using resting mycelia of Aspergillus parasiticus suspended in phosphate buffer. Malonate, iodoacetate, sodium arsenite, 2:4 dinitrophenol, sodium fluoride and p-aminosalicylate stimulated the incorporation at low concentrations and inhibited the same at high concentrations. p-Nitrobenzoic acid was inhibitory at all the concentrations tried. Fluoride, arsenite, arsenate and iodoacetate inhibited both growth and aflatoxin production when added directly to the growth medium. In general, there was a greater inhibition in growth medium than with the suspended mycelia.

摘要

利用悬浮于磷酸盐缓冲液中的寄生曲霉静止菌丝体，研究了多种代谢抑制剂对醋酸 -1-¹⁴C掺入黄曲霉毒素的影响。丙二酸、碘乙酸、亚砷酸钠、2,4-二硝基苯酚、氟化钠和对氨基水杨酸在低浓度时刺激掺入，在高浓度时抑制掺入。对硝基苯甲酸在所有试验浓度下均有抑制作用。当直接添加到生长培养基中时，氟化物、亚砷酸盐、砷酸盐和碘乙酸既抑制生长也抑制黄曲霉毒素的产生。一般来说，生长培养基中的抑制作用比悬浮菌丝体中的更大。