Griffiths E
J Gen Microbiol. 1975 Jun;88(2):345-54. doi: 10.1099/00221287-88-2-345.
The killing of Pasteurella septica by horse antiserum has features not previously associated with serum bactericidal reactions. The present work showed that lowering the pH from 7-4 to 6-8 abolished the action of antiserum. The bactericidal effect and the degradation of RNA seen when antiserum is added to P. septica growing in horse serum, were abolished at pH 6-8 in much the same way as when haem compounds were added to the system. Addition of chloramphenicol, rifampicin or puromycin to P. septica growing apparently normally in antiserum at pH 6-8 or in antiserum containing haem compounds led to rapid killing of the bacteria and to degration of their RNA. Addition of these antibiotics to P. septica growing in normal serum produced only bacteriostasis and did not induce RNA breakdown. In contrast, nalidixic acid, although inhibiting growth, did not induce rapid killing and RNA breakdown under the same conditions. These findings were unexpected and led to a reassessment of ideas concerning the mechanism of action of specific antiserum to P. septica. Although iron compounds clearly abolish the bactericidal based simply on an interference with bacterial iron supply is no longer sufficient. The process is more complex and must involve other factors.
马抗血清对败血巴氏杆菌的杀灭作用具有一些以前与血清杀菌反应无关的特征。目前的研究表明，将pH值从7.4降至6.8会消除抗血清的作用。当抗血清添加到在马血清中生长的败血巴氏杆菌时所观察到的杀菌效果和RNA降解，在pH值为6.8时以与向该系统中添加血红素化合物时大致相同的方式被消除。在pH值为6.8的抗血清中或在含有血红素化合物的抗血清中，向表面生长正常的败血巴氏杆菌添加氯霉素、利福平或嘌呤霉素会导致细菌迅速被杀死并使其RNA降解。向在正常血清中生长的败血巴氏杆菌添加这些抗生素仅产生抑菌作用，并未诱导RNA分解。相比之下，萘啶酸虽然抑制生长，但在相同条件下不会诱导细菌迅速被杀死和RNA分解。这些发现出乎意料，并导致重新评估关于抗败血巴氏杆菌特异性抗血清作用机制的观点。尽管铁化合物显然会消除杀菌作用，但仅仅基于对细菌铁供应的干扰已不再充分。这个过程更为复杂，必定涉及其他因素。
