Sim I S, Stephen J
J Gen Microbiol. 1975 Jul;89(1):11-25. doi: 10.1099/00221287-89-1-11.
Immunosorbents were derived from avid and non-avid sera raised in rabbits to multiple or single injections of chlamydiae passaged once or three times in HeLa cells after routine passage in eggs. Egg-derived suspensions of chlamydiae required pretreatment before application to immunosorbent columns; this was most conveniently done by fractionation on Sepharose 4B. Immunosorbents derived from avid serum had greater capacity than those from non-avid sera. However, organisms were desorbed in low yield from an avid immunosorbent, but in higher yields from non-avid immunosorbents. Even under the best conditions, the immunosorbents yielded suspensions of organisms still contaminated with egg antigens. Partially purified suspensions of chlamydiae were also treated with phospholipase C to yield suspensions of high purity.
免疫吸附剂源自兔子体内产生的抗血清和非抗血清，这些兔子经多次或单次注射衣原体后获得抗血清，所注射的衣原体是在鸡胚中常规传代后，再在HeLa细胞中传代一次或三次得到的。源自鸡胚的衣原体悬液在应用于免疫吸附柱之前需要进行预处理；最方便的做法是在琼脂糖4B上进行分级分离。源自抗血清的免疫吸附剂比源自非抗血清的免疫吸附剂具有更大的容量。然而，从抗免疫吸附剂上解吸出的生物体产量较低，而从非抗免疫吸附剂上解吸出的产量较高。即使在最佳条件下，免疫吸附剂产生的生物体悬液仍被鸡胚抗原污染。衣原体的部分纯化悬液也用磷脂酶C处理以产生高纯度的悬液。
