Howard L, Orenstein N S, King N W
Appl Microbiol. 1974 Jan;27(1):102-6. doi: 10.1128/am.27.1.102-106.1974.
Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate decrease in infectivity. For large-scale purification, infected yolk sac was centrifuged through Renografin solutions, resulting in greater than 60% recovery of starting chlamydial particles, but less than 1% recovery of the dry weight and protein.
将在鸡胚卵黄囊中培养的沙眼衣原体通过在连续等密度泛影葡胺密度梯度上离心进行纯化。一条浮力密度为1.20的衣原体颗粒带包含了70%的起始颗粒，并且电子显微镜显示几乎不存在污染的卵物质。在泛影葡胺梯度上离心仅导致感染性适度降低。对于大规模纯化，将感染的卵黄囊通过泛影葡胺溶液离心，起始衣原体颗粒的回收率大于60%，但干重和蛋白质的回收率小于1%。
