Department of Pathology, University of Medicine and Pharmacy, 38 Gheorghe Marinescu Street, Târgu-Mures 540000, Romania.
Pathol Res Pract. 2013 Sep;209(9):585-92. doi: 10.1016/j.prp.2013.06.012. Epub 2013 Jul 8.
We aimed to evaluate the expression and diagnostic value of five immunohistochemical markers (HBME-1, Galectin-3, CK19, CD56 and p63) in a very large series of unequivocal papillary thyroid carcinoma (PTC) cases, including both the classic (CPTC) and the follicular variant (FVPTC). We performed an immunohistochemical analysis on a tissue micro-array of 204 PTCs (98 CPTCs, 90 FVPTCs, and 16 other variants). HBME-1 was the most sensitive marker, staining 95.9% of CPTCs and 81.1% of FVPTCs. CD56, a marker whose expression is reduced or absent in thyroid carcinoma, revealed a negative, "malignant" profile in 93.9% of CPTCs and 73.3% of FVPTCs. Galectin-3, CK19 and p63 were positive in 64.7%, 45.6% and 6.9% of PTCs, respectively. The immunopanel consisting of HBME-1, CD56 and/or CK19 reached the highest sensitivity (95.6%). The co-expression of 2 or more proteins was observed in 88.2% of PTCs, with HBME-1 and CD56 being the most frequent positive association (79.4%). We report a new panel of antibodies consisting of HBME-1, CK19 and CD56 that was found to be highly sensitive for both CPTC and FVPTC. This panel could be recommended as a supplement to the morphological criteria in the diagnosis of difficult FVPTC cases.
我们旨在评估五种免疫组织化学标志物（HBME-1、Galectin-3、CK19、CD56 和 p63）在明确的甲状腺乳头状癌（PTC）病例中的表达和诊断价值，包括经典型（CPTC）和滤泡变体型（FVPTC）。我们对 204 例 PTC （98 例 CPTC、90 例 FVPTC 和 16 例其他变体）的组织微阵列进行了免疫组织化学分析。HBME-1 是最敏感的标志物，染色 CPTC 的 95.9%和 FVPTC 的 81.1%。CD56 是一种在甲状腺癌中表达减少或缺失的标志物，在 93.9%的 CPTC 和 73.3%的 FVPTC 中呈现阴性的“恶性”特征。Galectin-3、CK19 和 p63 的阳性率分别为 64.7%、45.6%和 6.9%。由 HBME-1、CD56 和/或 CK19 组成的免疫组套达到了最高的敏感性（95.6%）。2 种或更多种蛋白的共表达在 88.2%的 PTC 中观察到，其中 HBME-1 和 CD56 是最常见的阳性关联（79.4%）。我们报告了一个新的抗体组套，由 HBME-1、CK19 和 CD56 组成，对 CPTC 和 FVPTC 均具有高度敏感性。该组套可作为诊断困难 FVPTC 病例的形态学标准的补充。
